Field trial to evaluate the immunogenicity of pseudorabies virus vaccines with deletions for glycoproteins G and E

Lawrence D Firkins, R. M. Weigel, L. G. Biehl, E. C. Hahn

Research output: Contribution to journalArticlepeer-review

Abstract

Objective-To evaluate, under field conditions, the immunogenicity of 2 pseudorabies virus (PRV) vaccines (each with deletion of the gene for glycoprotein G [gG], and 1 with an additional deletion for glycoprotein E [gE]), particularly in the presence of maternal antibodies, and to investigate the effect of vaccination schedules in overcoming maternal antibody interference with vaccination. Sample Population-Two cohorts of 105 growing pigs each on a PRV-seronegative commercial swine farm where breeding stock had been vaccinated with a PRV vaccine containing deletions of genes for gG and gE. Procedure-Within each cohort, pigs were randomly assigned to 1 of 7 treatments groups. For each vaccine, vaccination was done at 8, 12, or 8 and 12 weeks of age. One group remained unvaccinated. Blood and nasal swab specimens were obtained at 8, 10, 12, 14, and 16 weeks of age, and the immune response was measured, by use of an ELISA. Results-In cohort 1, where prevalence of maternal antibodies at 8 weeks of age was lower, an immune response lasting until 16 weeks of age was induced in most pigs by either vaccine. In cohort 2, where prevalence of maternal antibodies at 8 weeks of age was higher, the gG-gE- vaccine elicited a lower immune response in the presence of maternal antibodies than did the gG- vaccine after single vaccination at 8 weeks of age. This maternal antibody interference with the response to vaccination was evident in serum and nasal mucosal antibodies. Conclusion-The gE deletion decreases the immunogenicity of PRV vaccine in the presence of maternal antibodies. Although evidence of maternal antibody interference for the gG- vaccine existed, its immunogencity was diminished less in the presence of maternal antibodies than that of the gG-gE- vaccine.

Original languageEnglish (US)
Pages (from-to)976-984
Number of pages9
JournalAmerican journal of veterinary research
Volume58
Issue number9
StatePublished - Sep 26 1997

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'Field trial to evaluate the immunogenicity of pseudorabies virus vaccines with deletions for glycoproteins G and E'. Together they form a unique fingerprint.

Cite this