Field-grown tobacco plants maintain robust growth while accumulating large quantities of a bacterial cellulase in chloroplasts

Jennifer A. Schmidt, Justin M. McGrath, Maureen R. Hanson, Stephen P Long, Beth A. Ahner

Research output: Contribution to journalArticle

Abstract

High accumulation of heterologous proteins expressed from the plastid genome has sometimes been reported to result in compromised plant phenotypes. Comparisons of transplastomic plants to wild-type (WT) are typically made in environmentally controlled chambers with relatively low light; little is known about the performance of such plants under field conditions. Here, we report on two plastid-engineered tobacco lines expressing the bacterial cellulase Cel6A. Field-grown plants producing Cel6A at ~20% of total soluble protein exhibit no loss in biomass or Rubisco content and only minor reductions in photosynthesis compared to WT. These experiments demonstrate that, when grown in the field, tobacco possesses sufficient metabolic flexibility to accommodate high levels of recombinant protein by increasing total protein synthesis and accumulation and/or by reallocating unneeded endogenous proteins. Based on current tobacco cultivation practices and readily achievable recombinant protein yields, we estimate that specific proteins could be obtained from field-grown transgenic tobacco plants at costs three orders of magnitude less than current cell culture methods.

Original languageEnglish (US)
Pages (from-to)715-721
Number of pages7
JournalNature Plants
Volume5
Issue number7
DOIs
StatePublished - Jul 1 2019

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endo-1,4-beta-glucanase
tobacco
chloroplasts
recombinant proteins
proteins
ribulose-bisphosphate carboxylase
plastids
cell culture
protein synthesis
photosynthesis
genetically modified organisms
phenotype
biomass
methodology

ASJC Scopus subject areas

  • Plant Science

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Field-grown tobacco plants maintain robust growth while accumulating large quantities of a bacterial cellulase in chloroplasts. / Schmidt, Jennifer A.; McGrath, Justin M.; Hanson, Maureen R.; Long, Stephen P; Ahner, Beth A.

In: Nature Plants, Vol. 5, No. 7, 01.07.2019, p. 715-721.

Research output: Contribution to journalArticle

Schmidt, Jennifer A. ; McGrath, Justin M. ; Hanson, Maureen R. ; Long, Stephen P ; Ahner, Beth A. / Field-grown tobacco plants maintain robust growth while accumulating large quantities of a bacterial cellulase in chloroplasts. In: Nature Plants. 2019 ; Vol. 5, No. 7. pp. 715-721.
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