FGF signaling is required for lens regeneration in Xenopus Laevis

Research output: Contribution to journalArticle

Abstract

In species of the frog genus Xenopus, lens regeneration occurs through a process of transdifferentiation, in which cornea epithelial cells presumably undergo dedifferentiation and subsequently redifferentiate to form a new lens. Experimental studies have shown that the retina provides the key signal required to trigger this process once the original lens is removed. A previous study showed that addition of an exogenous fibroblast growth factor (i.e., FGF1 protein) could initiate transdifferentiation of cornea epithelial cells in culture. To determine the role of FGF signaling in X. laevis lens regeneration, we have examined the presence of specific FGFs and their receptors (FGFRs) during this process and evaluated the necessity of FGFR signaling. Reverse transcriptase-polymerase chain reaction analyses reveal that a number of FGF family members are expressed in cornea epithelium and retinal tissues both before and during the process of lens regeneration. Of these, FGF1, FGF8, and FGF9 are expressed principally in retinal tissue and not in the cornea epithelium. Hence, these ligands could represent key signaling factors originating from the retina that trigger regeneration. The results of experiments using an in vitro eye culture system and an FGFR inhibitor (SU5402) suggest that FGFR signaling is required for lens regeneration in Xenopus.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalBiological Bulletin
Volume221
Issue number1
DOIs
StatePublished - Jan 1 2011

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Xenopus laevis
Lens
Lenses
Regeneration
Fibroblast Growth Factor Receptors
cornea
Cornea
Fibroblast Growth Factor 1
receptors
Xenopus
retina
Retina
epithelial cells
epithelium
Epithelium
Epithelial Cells
fibroblast growth factors
Fibroblast Growth Factors
Reverse Transcriptase Polymerase Chain Reaction
Anura

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

FGF signaling is required for lens regeneration in Xenopus Laevis. / Fukui, Lisa; Henry, Jonathan Joseph.

In: Biological Bulletin, Vol. 221, No. 1, 01.01.2011, p. 137-145.

Research output: Contribution to journalArticle

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