TY - JOUR
T1 - Feeding a Saccharomyces cerevisiae fermentation product improves udder health and immune response to a Streptococcus uberis mastitis challenge in mid-lactation dairy cows
AU - Vailati-Riboni, M.
AU - Coleman, D. N.
AU - Lopreiato, V.
AU - Alharthi, A.
AU - Bucktrout, R. E.
AU - Abdel-Hamied, E.
AU - Martinez-Cortes, I.
AU - Liang, Y.
AU - Trevisi, E.
AU - Yoon, I.
AU - Loor, J. J.
N1 - A. S. Alharthi received a fellowship from King Saud University to perform his PhD studies at the University of Illinois (Urbana). E. Abdel-Hamied was supported by a postdoctoral fellowship from the government of the Arab Republic of Egypt. Diamond V had a role in the study design and provided financial support to cover costs of animal use, data collection, and sample analysis.
We thank Adisseo (Alpharetta, GA) for the donation of Smartamine M, Ajinomoto Heartland Inc. (Chicago, IL) for the donation of Ajipro-L Generation 3; Dairy Nutrition Plus (Ralston, IA) for the donation of Soyplus; and Perdue AgriBusiness (Salisbury, MD) for the donation of ProVAAl2 AADvantage during the course of the experiment.
PY - 2021/12
Y1 - 2021/12
N2 - Background: We aimed to characterize the protective effects and the molecular mechanisms of action of a Saccharomyces cerevisiae fermentation product (NTK) in response to a mastitis challenge. Eighteen mid-lactation multiparous Holstein cows (n = 9/group) were fed the control diet (CON) or CON supplemented with 19 g/d NTK for 45 d (phase 1, P1) and then infected in the right rear quarter with 2500 CFU of Streptococcus uberis (phase 2, P2). After 36-h, mammary gland and liver biopsies were collected and antibiotic treatment started until the end of P2 (9 d post challenge). Cows were then followed until day 75 (phase 3, P3). Milk yield (MY) and dry matter intake (DMI) were recorded daily. Milk samples for somatic cell score were collected, and rectal and udder temperature, heart and respiration rate were recorded during the challenge period (P2) together with blood samples for metabolite and immune function analyses. Data were analyzed by phase using the PROC MIXED procedure in SAS. Biopsies were used for transcriptomic analysis via RNA-sequencing, followed by pathway analysis. Results: DMI and MY were not affected by diet in P1, but an interaction with time was recorded in P2 indicating a better recovery from the challenge in NTK compared with CON. NTK reduced rectal temperature, somatic cell score, and temperature of the infected quarter during the challenge. Transcriptome data supported these findings, as NTK supplementation upregulated mammary genes related to immune cell antibacterial function (e.g., CATHL4, NOS2), epithelial tissue protection (e.g. IL17C), and anti-inflammatory activity (e.g., ATF3, BAG3, IER3, G-CSF, GRO1, ZFAND2A). Pathway analysis indicated upregulation of tumor necrosis factor α, heat shock protein response, and p21 related pathways in the response to mastitis in NTK cows. Other pathways for detoxification and cytoprotection functions along with the tight junction pathway were also upregulated in NTK-fed cows. Conclusions: Overall, results highlighted molecular networks involved in the protective effect of NTK prophylactic supplementation on udder health during a subclinical mastitic event.
AB - Background: We aimed to characterize the protective effects and the molecular mechanisms of action of a Saccharomyces cerevisiae fermentation product (NTK) in response to a mastitis challenge. Eighteen mid-lactation multiparous Holstein cows (n = 9/group) were fed the control diet (CON) or CON supplemented with 19 g/d NTK for 45 d (phase 1, P1) and then infected in the right rear quarter with 2500 CFU of Streptococcus uberis (phase 2, P2). After 36-h, mammary gland and liver biopsies were collected and antibiotic treatment started until the end of P2 (9 d post challenge). Cows were then followed until day 75 (phase 3, P3). Milk yield (MY) and dry matter intake (DMI) were recorded daily. Milk samples for somatic cell score were collected, and rectal and udder temperature, heart and respiration rate were recorded during the challenge period (P2) together with blood samples for metabolite and immune function analyses. Data were analyzed by phase using the PROC MIXED procedure in SAS. Biopsies were used for transcriptomic analysis via RNA-sequencing, followed by pathway analysis. Results: DMI and MY were not affected by diet in P1, but an interaction with time was recorded in P2 indicating a better recovery from the challenge in NTK compared with CON. NTK reduced rectal temperature, somatic cell score, and temperature of the infected quarter during the challenge. Transcriptome data supported these findings, as NTK supplementation upregulated mammary genes related to immune cell antibacterial function (e.g., CATHL4, NOS2), epithelial tissue protection (e.g. IL17C), and anti-inflammatory activity (e.g., ATF3, BAG3, IER3, G-CSF, GRO1, ZFAND2A). Pathway analysis indicated upregulation of tumor necrosis factor α, heat shock protein response, and p21 related pathways in the response to mastitis in NTK cows. Other pathways for detoxification and cytoprotection functions along with the tight junction pathway were also upregulated in NTK-fed cows. Conclusions: Overall, results highlighted molecular networks involved in the protective effect of NTK prophylactic supplementation on udder health during a subclinical mastitic event.
KW - Dairy cow
KW - Mastitis
KW - RNA-sequencing
KW - Saccharomyces cerevisiae fermentation product
KW - Udder health
UR - https://www.scopus.com/pages/publications/85104017875
UR - https://www.scopus.com/pages/publications/85104017875#tab=citedBy
U2 - 10.1186/s40104-021-00560-8
DO - 10.1186/s40104-021-00560-8
M3 - Article
C2 - 33827684
AN - SCOPUS:85104017875
SN - 1674-9782
VL - 12
JO - Journal of Animal Science and Biotechnology
JF - Journal of Animal Science and Biotechnology
IS - 1
M1 - 62
ER -