Fc-directed antibody conjugation of magnetic nanoparticles for enhanced molecular targeting

Robabeh Rezaeipoor; Renu John; Steven G. Adie; Eric J. Chaney; Marina Marjanovic; Amy L. Oldenburg; Stephanie A. Rinne; Stephen A. Boppart

doi:10.1142/S1793545809000693

Fc-directed antibody conjugation of magnetic nanoparticles for enhanced molecular targeting

Robabeh Rezaeipoor, Renu John, Steven G. Adie, Eric J. Chaney, Marina Marjanovic, Amy L. Oldenburg, Stephanie A. Rinne, Stephen A. Boppart

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, we report the fabrication of engineered iron oxide magnetic nanoparticles (MNPs) functionalized with anti-human epidermal growth factor receptor type 2 (HER2) antibody to target the tumor antigen HER2. The Fc-directed conjugation of antibodies to the MNPs aids their efficient immunospecific targeting through free Fab portions. The directional specificity of conjugation was verified on a macrophage cell line. Immunofluorescence studies on macrophages treated with functionalized MNPs and free anti-HER2 antibody revealed that the antibody molecules bind to the MNPs predominantly through their Fc portion. Different cell lines with different HER2 expression levels were used to test the specificity of our functionalized nanoprobe for molecular targeting applications. The results of cell line targeting demonstrate that these engineered MNPs are able to differentiate between cell lines with different levels of HER2 expression.

Original language	English (US)
Pages (from-to)	387-396
Number of pages	10
Journal	Journal of Innovative Optical Health Sciences
Volume	2
Issue number	4
DOIs	https://doi.org/10.1142/S1793545809000693
State	Published - Oct 2009

Keywords

cancer
molecular imaging
Nanoprobe

ASJC Scopus subject areas

Biomedical Engineering
Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Medicine (miscellaneous)

Online availability

10.1142/S1793545809000693

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title = "Fc-directed antibody conjugation of magnetic nanoparticles for enhanced molecular targeting",

abstract = "In this study, we report the fabrication of engineered iron oxide magnetic nanoparticles (MNPs) functionalized with anti-human epidermal growth factor receptor type 2 (HER2) antibody to target the tumor antigen HER2. The Fc-directed conjugation of antibodies to the MNPs aids their efficient immunospecific targeting through free Fab portions. The directional specificity of conjugation was verified on a macrophage cell line. Immunofluorescence studies on macrophages treated with functionalized MNPs and free anti-HER2 antibody revealed that the antibody molecules bind to the MNPs predominantly through their Fc portion. Different cell lines with different HER2 expression levels were used to test the specificity of our functionalized nanoprobe for molecular targeting applications. The results of cell line targeting demonstrate that these engineered MNPs are able to differentiate between cell lines with different levels of HER2 expression.",

keywords = "cancer, molecular imaging, Nanoprobe",

author = "Robabeh Rezaeipoor and Renu John and Adie, {Steven G.} and Chaney, {Eric J.} and Marina Marjanovic and Oldenburg, {Amy L.} and Rinne, {Stephanie A.} and Boppart, {Stephen A.}",

note = "Funding Information: We would like to thank Scott Robinson from the Imaging Technology Group at the Beckman Institute for Advanced Science and Technology for his assistance with TEM analysis. This research was supported in part by grants from the National Institutes of Health (Roadmap Initiative, NIBIB, R21 EB005321, S.A.B., and NIBIB, R01 EB005221, S.A.B.).",

year = "2009",

month = oct,

doi = "10.1142/S1793545809000693",

language = "English (US)",

volume = "2",

pages = "387--396",

journal = "Journal of Innovative Optical Health Sciences",

issn = "1793-5458",

publisher = "World Scientific Publishing Co. Pte Ltd",

number = "4",

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TY - JOUR

T1 - Fc-directed antibody conjugation of magnetic nanoparticles for enhanced molecular targeting

AU - Rezaeipoor, Robabeh

AU - John, Renu

AU - Adie, Steven G.

AU - Chaney, Eric J.

AU - Marjanovic, Marina

AU - Oldenburg, Amy L.

AU - Rinne, Stephanie A.

AU - Boppart, Stephen A.

N1 - Funding Information: We would like to thank Scott Robinson from the Imaging Technology Group at the Beckman Institute for Advanced Science and Technology for his assistance with TEM analysis. This research was supported in part by grants from the National Institutes of Health (Roadmap Initiative, NIBIB, R21 EB005321, S.A.B., and NIBIB, R01 EB005221, S.A.B.).

PY - 2009/10

Y1 - 2009/10

N2 - In this study, we report the fabrication of engineered iron oxide magnetic nanoparticles (MNPs) functionalized with anti-human epidermal growth factor receptor type 2 (HER2) antibody to target the tumor antigen HER2. The Fc-directed conjugation of antibodies to the MNPs aids their efficient immunospecific targeting through free Fab portions. The directional specificity of conjugation was verified on a macrophage cell line. Immunofluorescence studies on macrophages treated with functionalized MNPs and free anti-HER2 antibody revealed that the antibody molecules bind to the MNPs predominantly through their Fc portion. Different cell lines with different HER2 expression levels were used to test the specificity of our functionalized nanoprobe for molecular targeting applications. The results of cell line targeting demonstrate that these engineered MNPs are able to differentiate between cell lines with different levels of HER2 expression.

AB - In this study, we report the fabrication of engineered iron oxide magnetic nanoparticles (MNPs) functionalized with anti-human epidermal growth factor receptor type 2 (HER2) antibody to target the tumor antigen HER2. The Fc-directed conjugation of antibodies to the MNPs aids their efficient immunospecific targeting through free Fab portions. The directional specificity of conjugation was verified on a macrophage cell line. Immunofluorescence studies on macrophages treated with functionalized MNPs and free anti-HER2 antibody revealed that the antibody molecules bind to the MNPs predominantly through their Fc portion. Different cell lines with different HER2 expression levels were used to test the specificity of our functionalized nanoprobe for molecular targeting applications. The results of cell line targeting demonstrate that these engineered MNPs are able to differentiate between cell lines with different levels of HER2 expression.

KW - cancer

KW - molecular imaging

KW - Nanoprobe

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Fc-directed antibody conjugation of magnetic nanoparticles for enhanced molecular targeting

Abstract

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