Fatal ranavirus infection in a group of zoo-housed meller's chameleons (trioceros melleri)

Lauren B. Peiffer, Samantha Sander, Kathleen Gabrielson, Allan P. Pessier, Matthew C Allender, Thomas Waltzek, Kuttichantran Subramaniam, Natalie Stilwell, Laura Adamovicz, Ellen Bronson, Lisa M. Mangus

Research output: Contribution to journalArticle

Abstract

A group of five juvenile Meller's chameleons (Trioceros melleri) experienced 100% mortality over a period of 1 mo due to ranavirus infection. The index case was found dead without premonitory signs. The three subsequent cases presented with nonspecific clinical signs (lethargy, decreased appetite, ocular discharge) and were ultimately euthanatized. The final case died after initially presenting with skin lesions. Postmortem examination revealed thin body condition in all five animals and mild coelomic effusion and petechiae affecting the tongue and kidneys of one animal. Microscopically, all animals had multifocal necrosis of the spleen, liver, and kidney; four of five animals had necrosis of the nasal cavity; and two of five had necrosis of adrenal tissue, bone marrow, and skin. Numerous basophilic intracytoplasmic inclusions were present in the liver of all animals and nasal mucosa of three of the five animals. Consensus polymerase chain reaction for herpesvirus and adenovirus were negative, whereas ranavirus quantitative polymerase chain reaction was positive. Virus isolation followed by whole genome sequencing and Bayesian phylogenetic analysis classified the isolates as a strain of frog virus 3 (FV3) most closely related to an FV3 isolate responsible for a previous outbreak in the zoo's eastern box turtle (Terrapene carolina carolina) group. This case series documents the first known occurrence of ranavirus-associated disease in chameleons and demonstrates the potential for interspecies transmission between chelonian and squamate reptiles.

Original languageEnglish (US)
Pages (from-to)696-705
Number of pages10
JournalJournal of Zoo and Wildlife Medicine
Volume50
Issue number3
DOIs
StatePublished - Sep 23 2019

Fingerprint

Ranavirus
Chamaeleonidae
Lizards
zoos
Frog virus 3
Infection
infection
animals
necrosis
Necrosis
kidneys
Kidney
nasal mucosa
Polymerase Chain Reaction
Lethargy
Skin
liver
Turtles
Reptiles
Purpura

Keywords

  • Frog virus 3
  • Interspecies transmission
  • Viral sequencing

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Fatal ranavirus infection in a group of zoo-housed meller's chameleons (trioceros melleri). / Peiffer, Lauren B.; Sander, Samantha; Gabrielson, Kathleen; Pessier, Allan P.; Allender, Matthew C; Waltzek, Thomas; Subramaniam, Kuttichantran; Stilwell, Natalie; Adamovicz, Laura; Bronson, Ellen; Mangus, Lisa M.

In: Journal of Zoo and Wildlife Medicine, Vol. 50, No. 3, 23.09.2019, p. 696-705.

Research output: Contribution to journalArticle

Peiffer, LB, Sander, S, Gabrielson, K, Pessier, AP, Allender, MC, Waltzek, T, Subramaniam, K, Stilwell, N, Adamovicz, L, Bronson, E & Mangus, LM 2019, 'Fatal ranavirus infection in a group of zoo-housed meller's chameleons (trioceros melleri)', Journal of Zoo and Wildlife Medicine, vol. 50, no. 3, pp. 696-705. https://doi.org/10.1638/2018-0044
Peiffer, Lauren B. ; Sander, Samantha ; Gabrielson, Kathleen ; Pessier, Allan P. ; Allender, Matthew C ; Waltzek, Thomas ; Subramaniam, Kuttichantran ; Stilwell, Natalie ; Adamovicz, Laura ; Bronson, Ellen ; Mangus, Lisa M. / Fatal ranavirus infection in a group of zoo-housed meller's chameleons (trioceros melleri). In: Journal of Zoo and Wildlife Medicine. 2019 ; Vol. 50, No. 3. pp. 696-705.
@article{69d775c9b0184c9d894177da97fc3a3d,
title = "Fatal ranavirus infection in a group of zoo-housed meller's chameleons (trioceros melleri)",
abstract = "A group of five juvenile Meller's chameleons (Trioceros melleri) experienced 100{\%} mortality over a period of 1 mo due to ranavirus infection. The index case was found dead without premonitory signs. The three subsequent cases presented with nonspecific clinical signs (lethargy, decreased appetite, ocular discharge) and were ultimately euthanatized. The final case died after initially presenting with skin lesions. Postmortem examination revealed thin body condition in all five animals and mild coelomic effusion and petechiae affecting the tongue and kidneys of one animal. Microscopically, all animals had multifocal necrosis of the spleen, liver, and kidney; four of five animals had necrosis of the nasal cavity; and two of five had necrosis of adrenal tissue, bone marrow, and skin. Numerous basophilic intracytoplasmic inclusions were present in the liver of all animals and nasal mucosa of three of the five animals. Consensus polymerase chain reaction for herpesvirus and adenovirus were negative, whereas ranavirus quantitative polymerase chain reaction was positive. Virus isolation followed by whole genome sequencing and Bayesian phylogenetic analysis classified the isolates as a strain of frog virus 3 (FV3) most closely related to an FV3 isolate responsible for a previous outbreak in the zoo's eastern box turtle (Terrapene carolina carolina) group. This case series documents the first known occurrence of ranavirus-associated disease in chameleons and demonstrates the potential for interspecies transmission between chelonian and squamate reptiles.",
keywords = "Frog virus 3, Interspecies transmission, Viral sequencing",
author = "Peiffer, {Lauren B.} and Samantha Sander and Kathleen Gabrielson and Pessier, {Allan P.} and Allender, {Matthew C} and Thomas Waltzek and Kuttichantran Subramaniam and Natalie Stilwell and Laura Adamovicz and Ellen Bronson and Mangus, {Lisa M.}",
year = "2019",
month = "9",
day = "23",
doi = "10.1638/2018-0044",
language = "English (US)",
volume = "50",
pages = "696--705",
journal = "Journal of Zoo and Wildlife Medicine",
issn = "1042-7260",
publisher = "American Association of Zoo Veterinarians",
number = "3",

}

TY - JOUR

T1 - Fatal ranavirus infection in a group of zoo-housed meller's chameleons (trioceros melleri)

AU - Peiffer, Lauren B.

AU - Sander, Samantha

AU - Gabrielson, Kathleen

AU - Pessier, Allan P.

AU - Allender, Matthew C

AU - Waltzek, Thomas

AU - Subramaniam, Kuttichantran

AU - Stilwell, Natalie

AU - Adamovicz, Laura

AU - Bronson, Ellen

AU - Mangus, Lisa M.

PY - 2019/9/23

Y1 - 2019/9/23

N2 - A group of five juvenile Meller's chameleons (Trioceros melleri) experienced 100% mortality over a period of 1 mo due to ranavirus infection. The index case was found dead without premonitory signs. The three subsequent cases presented with nonspecific clinical signs (lethargy, decreased appetite, ocular discharge) and were ultimately euthanatized. The final case died after initially presenting with skin lesions. Postmortem examination revealed thin body condition in all five animals and mild coelomic effusion and petechiae affecting the tongue and kidneys of one animal. Microscopically, all animals had multifocal necrosis of the spleen, liver, and kidney; four of five animals had necrosis of the nasal cavity; and two of five had necrosis of adrenal tissue, bone marrow, and skin. Numerous basophilic intracytoplasmic inclusions were present in the liver of all animals and nasal mucosa of three of the five animals. Consensus polymerase chain reaction for herpesvirus and adenovirus were negative, whereas ranavirus quantitative polymerase chain reaction was positive. Virus isolation followed by whole genome sequencing and Bayesian phylogenetic analysis classified the isolates as a strain of frog virus 3 (FV3) most closely related to an FV3 isolate responsible for a previous outbreak in the zoo's eastern box turtle (Terrapene carolina carolina) group. This case series documents the first known occurrence of ranavirus-associated disease in chameleons and demonstrates the potential for interspecies transmission between chelonian and squamate reptiles.

AB - A group of five juvenile Meller's chameleons (Trioceros melleri) experienced 100% mortality over a period of 1 mo due to ranavirus infection. The index case was found dead without premonitory signs. The three subsequent cases presented with nonspecific clinical signs (lethargy, decreased appetite, ocular discharge) and were ultimately euthanatized. The final case died after initially presenting with skin lesions. Postmortem examination revealed thin body condition in all five animals and mild coelomic effusion and petechiae affecting the tongue and kidneys of one animal. Microscopically, all animals had multifocal necrosis of the spleen, liver, and kidney; four of five animals had necrosis of the nasal cavity; and two of five had necrosis of adrenal tissue, bone marrow, and skin. Numerous basophilic intracytoplasmic inclusions were present in the liver of all animals and nasal mucosa of three of the five animals. Consensus polymerase chain reaction for herpesvirus and adenovirus were negative, whereas ranavirus quantitative polymerase chain reaction was positive. Virus isolation followed by whole genome sequencing and Bayesian phylogenetic analysis classified the isolates as a strain of frog virus 3 (FV3) most closely related to an FV3 isolate responsible for a previous outbreak in the zoo's eastern box turtle (Terrapene carolina carolina) group. This case series documents the first known occurrence of ranavirus-associated disease in chameleons and demonstrates the potential for interspecies transmission between chelonian and squamate reptiles.

KW - Frog virus 3

KW - Interspecies transmission

KW - Viral sequencing

UR - http://www.scopus.com/inward/record.url?scp=85072633742&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072633742&partnerID=8YFLogxK

U2 - 10.1638/2018-0044

DO - 10.1638/2018-0044

M3 - Article

AN - SCOPUS:85072633742

VL - 50

SP - 696

EP - 705

JO - Journal of Zoo and Wildlife Medicine

JF - Journal of Zoo and Wildlife Medicine

SN - 1042-7260

IS - 3

ER -