Fast phase reconstruction in white light diffraction phase microscopy

Hoa V. Pham, Christopher Edwards, Lynford L. Goddard, Gabriel Popescu

Research output: Contribution to journalArticle

Abstract

In off-axis interferometry, we usually have to deal with the unwrapping process, which is very computationally intensive and prevents us from real time phase reconstruction. The wrapping problem usually occurs when imaging thick objects, which introduce phase shifts of more than 2-radians. However, in off-axis interferometry, the nonzero angle of interference of the two beams creates a ramp in the phase across the image that can produce phase wrapping errors. In this paper, we propose a simple technique that avoids the need for the unwrapping step in reconstructing quantitative phase images in white light diffraction phase microscopy of thin samples. We show that this approach can improve significantly the phase reconstruction speed and allow high impact applications, such as real-time blood testing.

Original languageEnglish (US)
Pages (from-to)A97-A101
JournalApplied Optics
Volume52
Issue number1
DOIs
StatePublished - Jan 1 2013

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Interferometry
Microscopic examination
interferometry
Diffraction
microscopy
ramps
diffraction
Phase shift
blood
Blood
phase shift
interference
Imaging techniques
Testing

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

Cite this

Fast phase reconstruction in white light diffraction phase microscopy. / Pham, Hoa V.; Edwards, Christopher; Goddard, Lynford L.; Popescu, Gabriel.

In: Applied Optics, Vol. 52, No. 1, 01.01.2013, p. A97-A101.

Research output: Contribution to journalArticle

Pham, Hoa V. ; Edwards, Christopher ; Goddard, Lynford L. ; Popescu, Gabriel. / Fast phase reconstruction in white light diffraction phase microscopy. In: Applied Optics. 2013 ; Vol. 52, No. 1. pp. A97-A101.
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