Fast, multiplane line-scan confocal microscopy using axially distributed slits

Jean Marc Tsang, Howard J. Gritton, Shoshana L. Das, Timothy D. Weber, Christopher S. Chen, Xue Han, Jerome Mertz

Research output: Contribution to journalArticlepeer-review


The inherent constraints on resolution, speed and field of view have hindered the development of high-speed, three-dimensional microscopy techniques over large scales. Here, we present a multiplane line-scan imaging strategy, which uses a series of axially distributed reflecting slits to probe different depths within a sample volume. Our technique enables the simultaneous imaging of an optically sectioned image stack with a single camera at frame rates of hundreds of hertz, without the need for axial scanning. We demonstrate the applicability of our system to monitor fast dynamics in biological samples by performing calcium imaging of neuronal activity in mouse brains and voltage imaging of cardiomyocytes in cardiac samples.

Original languageEnglish (US)
Pages (from-to)1339-1350
Number of pages12
JournalBiomedical Optics Express
Issue number3
StatePublished - Mar 1 2021

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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