TY - JOUR
T1 - Factor VIIa-tissue factor
T2 - Functional importance of protein-membrane interactions
AU - Morrissey, James H.
AU - Neuenschwander, Pierre F.
AU - Huang, Qiuling
AU - McCallum, Christine D.
AU - Su, Bixia
AU - Johnson, Arthur E.
PY - 1997/7
Y1 - 1997/7
N2 - The first enzyme in the blood clotting cascade consists of two distinct protein subunits: a catalytic subunit (factor VIIa; FVIIa) and an essential regulatory subunit (tissue factor; TF). FVIIa is a soluble plasma protease, while TF is a cell-surface, integral-membrane protein. The recently reported X-ray crystal structure of the complex of FVIIa and the isolated extracellular domain of TF has provided important insights into the protein-protein interactions that bind these two subunits together (1). Equally important in the functioning of the TF-FVIIa complex, but much less well understood, are a series of protein-phospholipid interactions involving TF, FVIIa, and the natural substrates of this enzyme, as well as protein-protein interactions important in substrate recognition by TF-FVIIa. Here we review recent studies on the membrane organization and role of protein-phospholipid interactions in the function of TF-FVIIa, the enzyme that triggers blood clotting in hemostasis and thrombosis.
AB - The first enzyme in the blood clotting cascade consists of two distinct protein subunits: a catalytic subunit (factor VIIa; FVIIa) and an essential regulatory subunit (tissue factor; TF). FVIIa is a soluble plasma protease, while TF is a cell-surface, integral-membrane protein. The recently reported X-ray crystal structure of the complex of FVIIa and the isolated extracellular domain of TF has provided important insights into the protein-protein interactions that bind these two subunits together (1). Equally important in the functioning of the TF-FVIIa complex, but much less well understood, are a series of protein-phospholipid interactions involving TF, FVIIa, and the natural substrates of this enzyme, as well as protein-protein interactions important in substrate recognition by TF-FVIIa. Here we review recent studies on the membrane organization and role of protein-phospholipid interactions in the function of TF-FVIIa, the enzyme that triggers blood clotting in hemostasis and thrombosis.
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U2 - 10.1055/s-0038-1657511
DO - 10.1055/s-0038-1657511
M3 - Article
C2 - 9198138
AN - SCOPUS:0030745169
SN - 0340-6245
VL - 78
SP - 112
EP - 116
JO - Thrombosis and Haemostasis
JF - Thrombosis and Haemostasis
IS - 1
ER -