Facile Removal of Leader Peptides from Lanthipeptides by Incorporation of a Hydroxy Acid

Noah A. Bindman, Silvia C. Bobeica, Wenshe R. Liu, Wilfred A. Van Der Donk

Research output: Contribution to journalArticlepeer-review

Abstract

The biosynthesis of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products typically involves a precursor peptide which contains a leader peptide that is important for the modification process, and that is removed in the final step by a protease. Genome mining efforts for new RiPPs are often hampered by the lack of a general method to remove the leader peptides. We describe here the incorporation of hydroxy acids into the precursor peptides in E. coli which results in connection of the leader peptide via an ester linkage that is readily cleaved by simple hydrolysis. We demonstrate the method for two lantibiotics, lacticin 481 and nukacin ISK-1.

Original languageEnglish (US)
Pages (from-to)6975-6978
Number of pages4
JournalJournal of the American Chemical Society
Volume137
Issue number22
DOIs
StatePublished - Jun 10 2015

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry

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