Facile purification and click labeling with 2-[18F]fluoroethyl azide using solid phase extraction cartridges

Dong Zhou, Wenhua Chu, Xin Peng, Jonathan McConathy, Robert H. Mach, John A. Katzenellenbogen

Research output: Contribution to journalArticlepeer-review


A facile method was developed to purify 2-[18F]fluoroethyl azide ([18F]FEA) using a C18 cartridge and an Oasis® HLB cartridge in series, in which [18F]FEA was exclusively trapped on the HLB cartridge. [18F]FEA can be eluted for reactions in solution; alternatively click labeling can be carried out on the HLB cartridge itself by loading an alkyne substrate and copper (I) catalyst dissolved in DMF onto the cartridge. This solid phase extraction methodology for purification and click labeling with [18F]FEA, either in solution or on the cartridge, is safe, simple, reproducible in high yield, and compatible with automated synthesis of 18F-labeled PET tracers.

Original languageEnglish (US)
Pages (from-to)952-954
Number of pages3
JournalTetrahedron Letters
Issue number7
StatePublished - Feb 11 2015


  • 2-Fluoroethyl azide
  • Click chemistry
  • Fluorine-18
  • Positron emission tomography
  • Radiolabeling
  • Solid phase extraction

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry


Dive into the research topics of 'Facile purification and click labeling with 2-[<sup>18</sup>F]fluoroethyl azide using solid phase extraction cartridges'. Together they form a unique fingerprint.

Cite this