TY - JOUR
T1 - Facile and gentle method for quantitative lysis of Escherichia coli and Salmonella typhimurium
AU - Crabtree, S.
AU - Cronan, J. E.
PY - 1984
Y1 - 1984
N2 - Garrett et al. (Mol. Gen. Genet. 182:326-331, 1981) constructed strains of Escherichia coli harboring derivatives of plasmid pBR322 that carry the lysis genes (S, R, and R(z)) of phage lambda. The plasmid construction placed the genes under control of the lactose operon operator-promotor (and thus of lac repressor). Induction of E. coli strains carrying these plasmids resulted in rapid lysis of the culture unless the S gene was defective, in which case the cells grew normally. A freeze-thaw treatment of induced cells carrying an S- plasmid gave quantitative lysis of either E. coli or Salmonella typhimurium cells under exceptionally gentle conditions. The method was equally effective on exponential phase cells and stationary phase cells and was readily extended to a large number of independent cultures.
AB - Garrett et al. (Mol. Gen. Genet. 182:326-331, 1981) constructed strains of Escherichia coli harboring derivatives of plasmid pBR322 that carry the lysis genes (S, R, and R(z)) of phage lambda. The plasmid construction placed the genes under control of the lactose operon operator-promotor (and thus of lac repressor). Induction of E. coli strains carrying these plasmids resulted in rapid lysis of the culture unless the S gene was defective, in which case the cells grew normally. A freeze-thaw treatment of induced cells carrying an S- plasmid gave quantitative lysis of either E. coli or Salmonella typhimurium cells under exceptionally gentle conditions. The method was equally effective on exponential phase cells and stationary phase cells and was readily extended to a large number of independent cultures.
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U2 - 10.1128/jb.158.1.354-356.1984
DO - 10.1128/jb.158.1.354-356.1984
M3 - Article
C2 - 6232260
AN - SCOPUS:0021243325
VL - 158
SP - 354
EP - 356
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 1
ER -