Facile and gentle method for quantitative lysis of Escherichia coli and Salmonella typhimurium

S. Crabtree, J. E. Cronan

Research output: Contribution to journalArticlepeer-review

Abstract

Garrett et al. (Mol. Gen. Genet. 182:326-331, 1981) constructed strains of Escherichia coli harboring derivatives of plasmid pBR322 that carry the lysis genes (S, R, and R(z)) of phage lambda. The plasmid construction placed the genes under control of the lactose operon operator-promotor (and thus of lac repressor). Induction of E. coli strains carrying these plasmids resulted in rapid lysis of the culture unless the S gene was defective, in which case the cells grew normally. A freeze-thaw treatment of induced cells carrying an S- plasmid gave quantitative lysis of either E. coli or Salmonella typhimurium cells under exceptionally gentle conditions. The method was equally effective on exponential phase cells and stationary phase cells and was readily extended to a large number of independent cultures.

Original languageEnglish (US)
Pages (from-to)354-356
Number of pages3
JournalJournal of bacteriology
Volume158
Issue number1
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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