Fabrication of cell penetrating peptide-conjugated bacterial cellulose nanofibrils with remarkable skin adhesion and water retention performance

Bacterial cellulose nanofibrils (BCNFs), possessing excellent biocompatibility as well as hygroscopicity, are receiving high interest as a biomaterial for biomedical and healthcare treatments, since they exert various interactions with tissues after surface modification with biochemicals such as peptides, proteins, and catechols. Herein, we report a BCNF-based skin adhesion system, wherein cell penetrating peptide (CPP)-conjugated BCNFs were employed to enhance the attraction to the skin under wet conditions. For this, we conjugated Bac7, a type of CPP, with the carboxylate of 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-oxidized BCNFs. We showed that Bac7-conjugated BCNFs exhibited both hydrophobic and electrostatic interactions with the cell membrane, which eventually led to the remarkable adhesion against the skin surface. Furthermore, we demonstrated that such tailored skin attraction played a key role in improving skin water retention.