Fabrication of biomimetically patterned surfaces and their application to probing plant-bacteria interactions

Boce Zhang, Yaguang Luo, Arne J. Pearlstein, Jesse Aplin, Yi Liu, Gary R. Bauchan, Gregory F. Payne, Qin Wang, Xiangwu Nou, Patricia D. Millner

Research output: Contribution to journalArticlepeer-review

Abstract

We have developed a two-step replica molding method for rapid fabrication of biomimetically patterned plant surfaces (BPS) using polydimethylsiloxane (PDMS-BPS) and agarose (AGAR-BPS). Beyond providing multiple identical specimens that faithfully reproduce leaf surface microstructure, this approach also offers unique chemical, physical, and biological features. PDMS-BPS provide good structural durability for SEM examination, have surface wettability comparable to plant surfaces for coating development, and allow for real-time monitoring of biosynthesis through incorporation into microfluidic devices. AGAR-BPS are compatible with bacterial growth, recovery, and quantification, and enable investigation of the effects of surface topography on spatially varying survival and inactivation of Escherichia coli cells during biocide treatment. Further development and application of these biomimetically patterned surfaces to study (and possibly modify) other aspects of plant-bacteria interactions can provide insight into controlling pathogen contamination in a wide range of applications.

Original languageEnglish (US)
Pages (from-to)12467-12478
Number of pages12
JournalACS Applied Materials and Interfaces
Volume6
Issue number15
DOIs
StatePublished - Aug 13 2014

Keywords

  • PDMS
  • agarose
  • biomimetically-patterned surfaces
  • plant-bacteria interaction
  • replica molding
  • surface topography

ASJC Scopus subject areas

  • Materials Science(all)

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