Extremely-rapid RNA detection in dot blots with digoxigenin-labeled RNA probes

Chih Yang Huang; Matsuo Kasai; Dennis E. Buetow

doi:10.1016/S1050-3862(98)00003-5

Extremely-rapid RNA detection in dot blots with digoxigenin-labeled RNA probes

Chih Yang Huang, Matsuo Kasai, Dennis E. Buetow

Molecular and Integrative Physiology

Research output: Contribution to journal › Article › peer-review

Abstract

An RNA dot blot technique is described which uses digoxigenin-labeled antisense RNA probes and a highly-sensitive chemiluminescent substrate. Detection is extremely rapid: 10 s for even low abundance mRNA.

Original language	English (US)
Pages (from-to)	109-112
Number of pages	4
Journal	Genetic Analysis - Biomolecular Engineering
Volume	14
Issue number	4
DOIs	https://doi.org/10.1016/S1050-3862(98)00003-5
State	Published - Oct 1998

Keywords

Dot blot
Mammalian tissue RNAs
Non-radioactive probes
Rapid detection

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Genetics

Online availability

10.1016/S1050-3862(98)00003-5

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title = "Extremely-rapid RNA detection in dot blots with digoxigenin-labeled RNA probes",

abstract = "An RNA dot blot technique is described which uses digoxigenin-labeled antisense RNA probes and a highly-sensitive chemiluminescent substrate. Detection is extremely rapid: 10 s for even low abundance mRNA.",

keywords = "Dot blot, Mammalian tissue RNAs, Non-radioactive probes, Rapid detection",

author = "Huang, {Chih Yang} and Matsuo Kasai and Buetow, {Dennis E.}",

year = "1998",

month = oct,

doi = "10.1016/S1050-3862(98)00003-5",

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volume = "14",

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journal = "Genetic Analysis - Biomolecular Engineering",

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AU - Huang, Chih Yang

AU - Kasai, Matsuo

AU - Buetow, Dennis E.

PY - 1998/10

Y1 - 1998/10

N2 - An RNA dot blot technique is described which uses digoxigenin-labeled antisense RNA probes and a highly-sensitive chemiluminescent substrate. Detection is extremely rapid: 10 s for even low abundance mRNA.

AB - An RNA dot blot technique is described which uses digoxigenin-labeled antisense RNA probes and a highly-sensitive chemiluminescent substrate. Detection is extremely rapid: 10 s for even low abundance mRNA.

KW - Dot blot

KW - Mammalian tissue RNAs

KW - Non-radioactive probes

KW - Rapid detection

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U2 - 10.1016/S1050-3862(98)00003-5

DO - 10.1016/S1050-3862(98)00003-5

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AN - SCOPUS:0031793670

SN - 1050-3862

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JO - Genetic Analysis - Biomolecular Engineering

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IS - 4

ER -

Extremely-rapid RNA detection in dot blots with digoxigenin-labeled RNA probes

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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