Extraction of newly immobilized 15n from an illinois mollisol using aqueous phenol

Xin Tao He, Frank J. Stevenson, Richard L. Mulvaney, Kenneth R. Kelley

Research output: Contribution to journalArticlepeer-review


Studies were conducted on an 15N-enriched Illinois Mollisol (Flanagan silt loam) to determine the extractability of newly immobilized N with aqueous phenol (45%, v/v). In comparison to mild extradants proposed as indexes of plant available N (i.e. 10 mw NaHCO3 and hot 10 mm CaCl2), phenol showed the highest selectivity for the immobilized N, both with regard to the amount of biomass N extracted and the magnitude of the extractability ratio (ER). Aqueous phenol was also more selective in removing the immobilized N than reagents used for extraction of soil organic matter (i.e. 150 mm Na4P2O7 and 100 mm KOH). Results with soils incubated for up to 120 days showed that the amount of 15N extracted with aqueous phenol fluctuated with time and was related to changes in the soil microbial population, e.g. ER was highest when the bacterial population was maximal. Aqueous phenol shows promise as a selective extradant of potentially-available (labile) organic N in soils.

Original languageEnglish (US)
Pages (from-to)857-862
Number of pages6
JournalSoil Biology and Biochemistry
Issue number6
StatePublished - 1988

ASJC Scopus subject areas

  • Microbiology
  • Soil Science


Dive into the research topics of 'Extraction of newly immobilized 15n from an illinois mollisol using aqueous phenol'. Together they form a unique fingerprint.

Cite this