TY - JOUR
T1 - Extracellular matrix protein composition dynamically changes during murine forelimb development
AU - Jacobson, Kathryn R.
AU - Saleh, Aya M.
AU - Lipp, Sarah N.
AU - Tian, Chengzhe
AU - Watson, Audrey R.
AU - Luetkemeyer, Callan M.
AU - Ocken, Alexander R.
AU - Spencer, Sabrina L.
AU - Kinzer-Ursem, Tamara L.
AU - Calve, Sarah
N1 - Publisher Copyright:
© 2024 The Author(s)
PY - 2024/2/16
Y1 - 2024/2/16
N2 - The extracellular matrix (ECM) is an integral part of multicellular organisms, connecting different cell layers and tissue types. During morphogenesis and growth, tissues undergo substantial reorganization. While it is intuitive that the ECM remodels in concert, little is known regarding how matrix composition and organization change during development. Here, we quantified ECM protein dynamics in the murine forelimb during appendicular musculoskeletal morphogenesis (embryonic days 11.5–14.5) using tissue fractionation, bioorthogonal non-canonical amino acid tagging, and mass spectrometry. Our analyses indicated that ECM protein (matrisome) composition in the embryonic forelimb changed as a function of development and growth, was distinct from other developing organs (brain), and was altered in a model of disease (osteogenesis imperfecta murine). Additionally, the tissue distribution for select matrisome was assessed via immunohistochemistry in the wild-type embryonic and postnatal musculoskeletal system. This resource will guide future research investigating the role of the matrisome during complex tissue development.
AB - The extracellular matrix (ECM) is an integral part of multicellular organisms, connecting different cell layers and tissue types. During morphogenesis and growth, tissues undergo substantial reorganization. While it is intuitive that the ECM remodels in concert, little is known regarding how matrix composition and organization change during development. Here, we quantified ECM protein dynamics in the murine forelimb during appendicular musculoskeletal morphogenesis (embryonic days 11.5–14.5) using tissue fractionation, bioorthogonal non-canonical amino acid tagging, and mass spectrometry. Our analyses indicated that ECM protein (matrisome) composition in the embryonic forelimb changed as a function of development and growth, was distinct from other developing organs (brain), and was altered in a model of disease (osteogenesis imperfecta murine). Additionally, the tissue distribution for select matrisome was assessed via immunohistochemistry in the wild-type embryonic and postnatal musculoskeletal system. This resource will guide future research investigating the role of the matrisome during complex tissue development.
KW - Biological sciences
KW - Developmental biology
KW - Natural sciences
UR - http://www.scopus.com/inward/record.url?scp=85183191588&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85183191588&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2024.108838
DO - 10.1016/j.isci.2024.108838
M3 - Article
C2 - 38303699
AN - SCOPUS:85183191588
SN - 2589-0042
VL - 27
JO - iScience
JF - iScience
IS - 2
M1 - 108838
ER -