Abstract
Voltage-driven activation of Kv channels results from conformational changes of four voltage sensor domains (VSDs) that surround the K+ selective pore domain. How the VSD helices rearrange during gating is an area of active research. Luminescence resonance energy transfer (LRET) is a powerful spectroscopic ruler uniquely suitable for addressing the conformational trajectory of these helices. Using a geometric analysis of numerous LRET measurements, we were able to estimate LRET probe positions relative to existing structural models. The experimental movement of helix S4 does not support a large 15-20 Å transmembrane "paddle-type" movement or a near-zero Å vertical "transporter-type" model. Rather, our measurements demonstrate a moderate S4 displacement of 10 ± 5 Å, with a vertical component of 5 ± 2 Å. The S3 segment moves 2 ± 1 Å in the opposite direction and is therefore not moving as an S3-S4 rigid body.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 98-109 |
| Number of pages | 12 |
| Journal | Neuron |
| Volume | 59 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 10 2008 |
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Keywords
- MOLNEURO
- PROTEINS
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Extent of Voltage Sensor Movement during Gating of Shaker K+ Channels. / Posson, David J.; Selvin, Paul R.
In: Neuron, Vol. 59, No. 1, 10.07.2008, p. 98-109.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extent of Voltage Sensor Movement during Gating of Shaker K+ Channels
AU - Posson, David J.
AU - Selvin, Paul R
PY - 2008/7/10
Y1 - 2008/7/10
N2 - Voltage-driven activation of Kv channels results from conformational changes of four voltage sensor domains (VSDs) that surround the K+ selective pore domain. How the VSD helices rearrange during gating is an area of active research. Luminescence resonance energy transfer (LRET) is a powerful spectroscopic ruler uniquely suitable for addressing the conformational trajectory of these helices. Using a geometric analysis of numerous LRET measurements, we were able to estimate LRET probe positions relative to existing structural models. The experimental movement of helix S4 does not support a large 15-20 Å transmembrane "paddle-type" movement or a near-zero Å vertical "transporter-type" model. Rather, our measurements demonstrate a moderate S4 displacement of 10 ± 5 Å, with a vertical component of 5 ± 2 Å. The S3 segment moves 2 ± 1 Å in the opposite direction and is therefore not moving as an S3-S4 rigid body.
AB - Voltage-driven activation of Kv channels results from conformational changes of four voltage sensor domains (VSDs) that surround the K+ selective pore domain. How the VSD helices rearrange during gating is an area of active research. Luminescence resonance energy transfer (LRET) is a powerful spectroscopic ruler uniquely suitable for addressing the conformational trajectory of these helices. Using a geometric analysis of numerous LRET measurements, we were able to estimate LRET probe positions relative to existing structural models. The experimental movement of helix S4 does not support a large 15-20 Å transmembrane "paddle-type" movement or a near-zero Å vertical "transporter-type" model. Rather, our measurements demonstrate a moderate S4 displacement of 10 ± 5 Å, with a vertical component of 5 ± 2 Å. The S3 segment moves 2 ± 1 Å in the opposite direction and is therefore not moving as an S3-S4 rigid body.
KW - MOLNEURO
KW - PROTEINS
UR - http://www.scopus.com/inward/record.url?scp=46149091062&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=46149091062&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2008.05.006
DO - 10.1016/j.neuron.2008.05.006
M3 - Article
C2 - 18614032
AN - SCOPUS:46149091062
VL - 59
SP - 98
EP - 109
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 1
ER -