Extensive genetic diversity in the Plasmodium falciparum Na +/H+ exchanger 1 transporter protein implicated in quinine resistance

Sumiti Vinayak; Mohammad Tauqeer Alam; Mala Upadhyay; Manoj K. Das; Vas Dev; Neeru Singh; Aditya P. Dash; Yagya D. Sharma

doi:10.1128/AAC.00317-07

Extensive genetic diversity in the Plasmodium falciparum Na ⁺/H⁺ exchanger 1 transporter protein implicated in quinine resistance

Sumiti Vinayak, Mohammad Tauqeer Alam, Mala Upadhyay, Manoj K. Das, Vas Dev, Neeru Singh, Aditya P. Dash, Yagya D. Sharma

Research output: Contribution to journal › Article › peer-review

Abstract

The Plasmodium falciparum Na⁺/H⁺ exchanger (Pfnhe-1) locus at chromosome 13 and another locus at chromosome 9 have recently been proposed to influence quinine resistance. Here, we sequenced the ms4760 locus of the Pfnhe-1 gene from 244 P. falciparum isolates collected from five different regions of India. A total of 16 different ms4760 alleles (with one to five DNNND repeats) were observed among these isolates. Interestingly, areas with a high prevalence of chloroquine and sulfadoxine-pyrimethamine resistance showed more Pfnhe-1 DNNND repeats compared to low drug resistance areas. The extent of genetic diversity at the ms4760 locus also varied from one region to another, with expected heterozygosity values ranging from 0.47 to 0.88.

Original language	English (US)
Pages (from-to)	4508-4511
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	51
Issue number	12
DOIs	https://doi.org/10.1128/AAC.00317-07
State	Published - Dec 2007
Externally published	Yes

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

Online availability

10.1128/AAC.00317-07

Library availability

Discover UIUC Full Text

Cite this

@article{590f81e1759c49748052432cdedc63c3,

title = "Extensive genetic diversity in the Plasmodium falciparum Na +/H+ exchanger 1 transporter protein implicated in quinine resistance",

abstract = "The Plasmodium falciparum Na+/H+ exchanger (Pfnhe-1) locus at chromosome 13 and another locus at chromosome 9 have recently been proposed to influence quinine resistance. Here, we sequenced the ms4760 locus of the Pfnhe-1 gene from 244 P. falciparum isolates collected from five different regions of India. A total of 16 different ms4760 alleles (with one to five DNNND repeats) were observed among these isolates. Interestingly, areas with a high prevalence of chloroquine and sulfadoxine-pyrimethamine resistance showed more Pfnhe-1 DNNND repeats compared to low drug resistance areas. The extent of genetic diversity at the ms4760 locus also varied from one region to another, with expected heterozygosity values ranging from 0.47 to 0.88.",

author = "Sumiti Vinayak and Alam, {Mohammad Tauqeer} and Mala Upadhyay and Das, {Manoj K.} and Vas Dev and Neeru Singh and Dash, {Aditya P.} and Sharma, {Yagya D.}",

year = "2007",

month = dec,

doi = "10.1128/AAC.00317-07",

language = "English (US)",

volume = "51",

pages = "4508--4511",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "12",

}

TY - JOUR

T1 - Extensive genetic diversity in the Plasmodium falciparum Na +/H+ exchanger 1 transporter protein implicated in quinine resistance

AU - Vinayak, Sumiti

AU - Alam, Mohammad Tauqeer

AU - Upadhyay, Mala

AU - Das, Manoj K.

AU - Dev, Vas

AU - Singh, Neeru

AU - Dash, Aditya P.

AU - Sharma, Yagya D.

PY - 2007/12

Y1 - 2007/12

N2 - The Plasmodium falciparum Na+/H+ exchanger (Pfnhe-1) locus at chromosome 13 and another locus at chromosome 9 have recently been proposed to influence quinine resistance. Here, we sequenced the ms4760 locus of the Pfnhe-1 gene from 244 P. falciparum isolates collected from five different regions of India. A total of 16 different ms4760 alleles (with one to five DNNND repeats) were observed among these isolates. Interestingly, areas with a high prevalence of chloroquine and sulfadoxine-pyrimethamine resistance showed more Pfnhe-1 DNNND repeats compared to low drug resistance areas. The extent of genetic diversity at the ms4760 locus also varied from one region to another, with expected heterozygosity values ranging from 0.47 to 0.88.

AB - The Plasmodium falciparum Na+/H+ exchanger (Pfnhe-1) locus at chromosome 13 and another locus at chromosome 9 have recently been proposed to influence quinine resistance. Here, we sequenced the ms4760 locus of the Pfnhe-1 gene from 244 P. falciparum isolates collected from five different regions of India. A total of 16 different ms4760 alleles (with one to five DNNND repeats) were observed among these isolates. Interestingly, areas with a high prevalence of chloroquine and sulfadoxine-pyrimethamine resistance showed more Pfnhe-1 DNNND repeats compared to low drug resistance areas. The extent of genetic diversity at the ms4760 locus also varied from one region to another, with expected heterozygosity values ranging from 0.47 to 0.88.

UR - http://www.scopus.com/inward/record.url?scp=36749018511&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36749018511&partnerID=8YFLogxK

U2 - 10.1128/AAC.00317-07

DO - 10.1128/AAC.00317-07

M3 - Article

C2 - 17923493

AN - SCOPUS:36749018511

SN - 0066-4804

VL - 51

SP - 4508

EP - 4511

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 12

ER -