Extended Multiplicative Signal Correction for Infrared Microspectroscopy of Heterogeneous Samples with Cylindrical Domains

Ilia L. Rasskazov, Rajveer Singh, P. Scott Carney, Rohit Bhargava

Research output: Contribution to journalArticle

Abstract

Optical scattering corrections are invoked to computationally distinguish between scattering and absorption contributions to recorded data in infrared (IR) microscopy, with a goal to obtain an absorption spectrum that is relatively free of the effects of sample morphology. Here, we present a modification of the extended multiplicative signal correction (EMSC) approach that allows for spectral recovery from fibers and cylindrical domains in heterogeneous samples. The developed theoretical approach is based on exact Mie theory for infinite cylinders. Although rigorous Mie theory implies utilization of comprehensive and time-consuming calculations, we propose to change the workflow of the original EMSC algorithm to minimize extensive calculations for each recorded spectrum at each iteration step. This makes the modified EMSC approach practical for routine use. First, we tested our approach using synthetic data derived from a rigorous model of scattering from cylinders in an IR microscope. Second, we applied the approach to Fourier transform IR (FT-IR) microspectroscopy data recorded from filamentous fungal and cellulose samples with pronounced fiber-like shapes. While the corrected spectra show greatly reduced baseline offsets and consistency, strongly absorbing regions of the spectrum require further refinement. The modified EMSC algorithm broadly mitigates the effects of scattering, offering a practical approach to more consistent and accurate spectra from cylindrical objects or heterogeneous samples with cylindrical domains.

Original languageEnglish (US)
Pages (from-to)859-869
Number of pages11
JournalApplied Spectroscopy
Volume73
Issue number8
DOIs
StatePublished - Aug 1 2019

Fingerprint

Scattering
Infrared radiation
Mie scattering
scattering
Fibers
Cellulose
fibers
Absorption spectra
Fourier transforms
Microscopic examination
Microscopes
cellulose
iteration
Recovery
recovery
microscopes
microscopy
absorption spectra

Keywords

  • Extended multiplicative signal correction
  • fibers
  • mid-infrared microspectroscopy
  • scattering

ASJC Scopus subject areas

  • Instrumentation
  • Spectroscopy

Cite this

Extended Multiplicative Signal Correction for Infrared Microspectroscopy of Heterogeneous Samples with Cylindrical Domains. / Rasskazov, Ilia L.; Singh, Rajveer; Carney, P. Scott; Bhargava, Rohit.

In: Applied Spectroscopy, Vol. 73, No. 8, 01.08.2019, p. 859-869.

Research output: Contribution to journalArticle

@article{12dbdc684f2f4243a8a00b43d0f31dc4,
title = "Extended Multiplicative Signal Correction for Infrared Microspectroscopy of Heterogeneous Samples with Cylindrical Domains",
abstract = "Optical scattering corrections are invoked to computationally distinguish between scattering and absorption contributions to recorded data in infrared (IR) microscopy, with a goal to obtain an absorption spectrum that is relatively free of the effects of sample morphology. Here, we present a modification of the extended multiplicative signal correction (EMSC) approach that allows for spectral recovery from fibers and cylindrical domains in heterogeneous samples. The developed theoretical approach is based on exact Mie theory for infinite cylinders. Although rigorous Mie theory implies utilization of comprehensive and time-consuming calculations, we propose to change the workflow of the original EMSC algorithm to minimize extensive calculations for each recorded spectrum at each iteration step. This makes the modified EMSC approach practical for routine use. First, we tested our approach using synthetic data derived from a rigorous model of scattering from cylinders in an IR microscope. Second, we applied the approach to Fourier transform IR (FT-IR) microspectroscopy data recorded from filamentous fungal and cellulose samples with pronounced fiber-like shapes. While the corrected spectra show greatly reduced baseline offsets and consistency, strongly absorbing regions of the spectrum require further refinement. The modified EMSC algorithm broadly mitigates the effects of scattering, offering a practical approach to more consistent and accurate spectra from cylindrical objects or heterogeneous samples with cylindrical domains.",
keywords = "Extended multiplicative signal correction, fibers, mid-infrared microspectroscopy, scattering",
author = "Rasskazov, {Ilia L.} and Rajveer Singh and Carney, {P. Scott} and Rohit Bhargava",
year = "2019",
month = "8",
day = "1",
doi = "10.1177/0003702819844528",
language = "English (US)",
volume = "73",
pages = "859--869",
journal = "Applied Spectroscopy",
issn = "0003-7028",
publisher = "Society for Applied Spectroscopy",
number = "8",

}

TY - JOUR

T1 - Extended Multiplicative Signal Correction for Infrared Microspectroscopy of Heterogeneous Samples with Cylindrical Domains

AU - Rasskazov, Ilia L.

AU - Singh, Rajveer

AU - Carney, P. Scott

AU - Bhargava, Rohit

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Optical scattering corrections are invoked to computationally distinguish between scattering and absorption contributions to recorded data in infrared (IR) microscopy, with a goal to obtain an absorption spectrum that is relatively free of the effects of sample morphology. Here, we present a modification of the extended multiplicative signal correction (EMSC) approach that allows for spectral recovery from fibers and cylindrical domains in heterogeneous samples. The developed theoretical approach is based on exact Mie theory for infinite cylinders. Although rigorous Mie theory implies utilization of comprehensive and time-consuming calculations, we propose to change the workflow of the original EMSC algorithm to minimize extensive calculations for each recorded spectrum at each iteration step. This makes the modified EMSC approach practical for routine use. First, we tested our approach using synthetic data derived from a rigorous model of scattering from cylinders in an IR microscope. Second, we applied the approach to Fourier transform IR (FT-IR) microspectroscopy data recorded from filamentous fungal and cellulose samples with pronounced fiber-like shapes. While the corrected spectra show greatly reduced baseline offsets and consistency, strongly absorbing regions of the spectrum require further refinement. The modified EMSC algorithm broadly mitigates the effects of scattering, offering a practical approach to more consistent and accurate spectra from cylindrical objects or heterogeneous samples with cylindrical domains.

AB - Optical scattering corrections are invoked to computationally distinguish between scattering and absorption contributions to recorded data in infrared (IR) microscopy, with a goal to obtain an absorption spectrum that is relatively free of the effects of sample morphology. Here, we present a modification of the extended multiplicative signal correction (EMSC) approach that allows for spectral recovery from fibers and cylindrical domains in heterogeneous samples. The developed theoretical approach is based on exact Mie theory for infinite cylinders. Although rigorous Mie theory implies utilization of comprehensive and time-consuming calculations, we propose to change the workflow of the original EMSC algorithm to minimize extensive calculations for each recorded spectrum at each iteration step. This makes the modified EMSC approach practical for routine use. First, we tested our approach using synthetic data derived from a rigorous model of scattering from cylinders in an IR microscope. Second, we applied the approach to Fourier transform IR (FT-IR) microspectroscopy data recorded from filamentous fungal and cellulose samples with pronounced fiber-like shapes. While the corrected spectra show greatly reduced baseline offsets and consistency, strongly absorbing regions of the spectrum require further refinement. The modified EMSC algorithm broadly mitigates the effects of scattering, offering a practical approach to more consistent and accurate spectra from cylindrical objects or heterogeneous samples with cylindrical domains.

KW - Extended multiplicative signal correction

KW - fibers

KW - mid-infrared microspectroscopy

KW - scattering

UR - http://www.scopus.com/inward/record.url?scp=85067044843&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067044843&partnerID=8YFLogxK

U2 - 10.1177/0003702819844528

DO - 10.1177/0003702819844528

M3 - Article

C2 - 31149835

AN - SCOPUS:85067044843

VL - 73

SP - 859

EP - 869

JO - Applied Spectroscopy

JF - Applied Spectroscopy

SN - 0003-7028

IS - 8

ER -