Expression profiling soybean response to Pseudomonas syringae reveals new defense-related genes and rapid HR-specific downregulation of photosynthesis

Jijun Zou, Sandra Rodriguez-Zas, Mihai Aldea, Min Li, Jin Zhu, Delkin O. Gonzalez, Lila O. Vodkin, Evan DeLucia, Steven J. Clough

Research output: Contribution to journalArticle

Abstract

Transcript profiling during susceptible (S) and hypersensitive response-associated resistance (R) interactions was determined in soybean (Glycine max). Pseudomonas syringae pv. glycinea carrying or lacking the avirulence gene avrB, was infiltrated into cultivar Williams 82. Leaf RNA was sampled at 2, 8, and 24 h postinoculation (hpi). Significant changes in transcript abundance were observed for 3,897 genes during the experiment at P ≤ 0.000005. Many of the genes showed a similar direction of increase or decrease in abundance in both the S and R responses, but the R response generally showed a significantly greater degree of differential expression. More than 25% of these responsive genes had not been previously reported as being associated with pathogen interactions, as 704 had no functional annotation and 378 had no homolog in National Center for Biotechnology Information databases. The highest number of transcriptional changes was noted at 8 hpi, including the downregulation of 94 chloroplast-associated genes specific to the R response. Photosynthetic measurements were consistent with an R-specific reduction in photosystem II operating efficiency (ΦPSII) that was apparent at 8 hpi for the R response with little effect in the S or control treatments. Imaging analyses suggest that the decreased ΦPSII was a result of physical damage to PSII reaction centers.

Original languageEnglish (US)
Pages (from-to)1161-1174
Number of pages14
JournalMolecular Plant-Microbe Interactions
Volume18
Issue number11
DOIs
StatePublished - Nov 1 2005

Fingerprint

Pseudomonas syringae
Photosynthesis
Soybeans
Down-Regulation
photosynthesis
soybeans
Genes
genes
Chloroplast Genes
Information Centers
Pseudomonas syringae pv. glycinea
Photosystem II Protein Complex
National Center for Biotechnology Information
Biotechnology
hypersensitive response
photosystem II
Databases
RNA
Glycine max
chloroplasts

Keywords

  • Compatible
  • Genomic
  • Incompatible
  • Oxidative burst

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

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title = "Expression profiling soybean response to Pseudomonas syringae reveals new defense-related genes and rapid HR-specific downregulation of photosynthesis",
abstract = "Transcript profiling during susceptible (S) and hypersensitive response-associated resistance (R) interactions was determined in soybean (Glycine max). Pseudomonas syringae pv. glycinea carrying or lacking the avirulence gene avrB, was infiltrated into cultivar Williams 82. Leaf RNA was sampled at 2, 8, and 24 h postinoculation (hpi). Significant changes in transcript abundance were observed for 3,897 genes during the experiment at P ≤ 0.000005. Many of the genes showed a similar direction of increase or decrease in abundance in both the S and R responses, but the R response generally showed a significantly greater degree of differential expression. More than 25{\%} of these responsive genes had not been previously reported as being associated with pathogen interactions, as 704 had no functional annotation and 378 had no homolog in National Center for Biotechnology Information databases. The highest number of transcriptional changes was noted at 8 hpi, including the downregulation of 94 chloroplast-associated genes specific to the R response. Photosynthetic measurements were consistent with an R-specific reduction in photosystem II operating efficiency (ΦPSII) that was apparent at 8 hpi for the R response with little effect in the S or control treatments. Imaging analyses suggest that the decreased ΦPSII was a result of physical damage to PSII reaction centers.",
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author = "Jijun Zou and Sandra Rodriguez-Zas and Mihai Aldea and Min Li and Jin Zhu and Gonzalez, {Delkin O.} and Vodkin, {Lila O.} and Evan DeLucia and Clough, {Steven J.}",
year = "2005",
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T1 - Expression profiling soybean response to Pseudomonas syringae reveals new defense-related genes and rapid HR-specific downregulation of photosynthesis

AU - Zou, Jijun

AU - Rodriguez-Zas, Sandra

AU - Aldea, Mihai

AU - Li, Min

AU - Zhu, Jin

AU - Gonzalez, Delkin O.

AU - Vodkin, Lila O.

AU - DeLucia, Evan

AU - Clough, Steven J.

PY - 2005/11/1

Y1 - 2005/11/1

N2 - Transcript profiling during susceptible (S) and hypersensitive response-associated resistance (R) interactions was determined in soybean (Glycine max). Pseudomonas syringae pv. glycinea carrying or lacking the avirulence gene avrB, was infiltrated into cultivar Williams 82. Leaf RNA was sampled at 2, 8, and 24 h postinoculation (hpi). Significant changes in transcript abundance were observed for 3,897 genes during the experiment at P ≤ 0.000005. Many of the genes showed a similar direction of increase or decrease in abundance in both the S and R responses, but the R response generally showed a significantly greater degree of differential expression. More than 25% of these responsive genes had not been previously reported as being associated with pathogen interactions, as 704 had no functional annotation and 378 had no homolog in National Center for Biotechnology Information databases. The highest number of transcriptional changes was noted at 8 hpi, including the downregulation of 94 chloroplast-associated genes specific to the R response. Photosynthetic measurements were consistent with an R-specific reduction in photosystem II operating efficiency (ΦPSII) that was apparent at 8 hpi for the R response with little effect in the S or control treatments. Imaging analyses suggest that the decreased ΦPSII was a result of physical damage to PSII reaction centers.

AB - Transcript profiling during susceptible (S) and hypersensitive response-associated resistance (R) interactions was determined in soybean (Glycine max). Pseudomonas syringae pv. glycinea carrying or lacking the avirulence gene avrB, was infiltrated into cultivar Williams 82. Leaf RNA was sampled at 2, 8, and 24 h postinoculation (hpi). Significant changes in transcript abundance were observed for 3,897 genes during the experiment at P ≤ 0.000005. Many of the genes showed a similar direction of increase or decrease in abundance in both the S and R responses, but the R response generally showed a significantly greater degree of differential expression. More than 25% of these responsive genes had not been previously reported as being associated with pathogen interactions, as 704 had no functional annotation and 378 had no homolog in National Center for Biotechnology Information databases. The highest number of transcriptional changes was noted at 8 hpi, including the downregulation of 94 chloroplast-associated genes specific to the R response. Photosynthetic measurements were consistent with an R-specific reduction in photosystem II operating efficiency (ΦPSII) that was apparent at 8 hpi for the R response with little effect in the S or control treatments. Imaging analyses suggest that the decreased ΦPSII was a result of physical damage to PSII reaction centers.

KW - Compatible

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KW - Incompatible

KW - Oxidative burst

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