Expression profiles of differentially regulated genes during the early stages of apple flower infection with Erwinia amylovora

Sujon Sarowar, Youfu Zhao, Ruth Elena Soria-Guerra, Shahjahan Ali, Danman Zheng, Dongping Wang, Schuyler S. Korban

Research output: Contribution to journalArticlepeer-review

Abstract

To identify genes involved in the response to the fire blight pathogen Erwinia amylovora in apple (Malus×domestica), expression profiles were investigated using an apple oligo (70-mer) array representing 40, 000 genes. Blossoms of a fire blight-susceptible apple cultivar Gala were collected from trees growing in the orchard, placed on a tray in the laboratory, and spray-inoculated with a suspension of E. amylovora at a concentration of 10 8 cfu ml -1. Uninoculated detached flowers served as controls at each time point. Expression profiles were captured at three different time points post-inoculation at 2, 8, and 24 h, together with those at 0 h (uninoculated). A total of about 3500 genes were found to be significantly modulated in response to at least one of the three time points. Among those, a total of 770, 855, and 1002 genes were up-regulated, by 2-fold, at 2, 8, and 24 h following inoculation, respectively; while, 748, 1024, and 1455 genes were down-regulated, by 2-fold, at 2, 8, and 24 h following inoculation, respectively. Over the three time points post-inoculation, 365 genes were commonly up-regulated and 374 genes were commonly down-regulated. Both sets of genes were classified based on their functional categories. The majority of up-regulated genes were involved in metabolism, signal transduction, signalling, transport, and stress response. A number of transcripts encoding proteins/enzymes known to be up-regulated under particular biotic and abiotic stress were also up-regulated following E. amylovora treatment. Those up-or down-regulated genes encode transcription factors, signaling components, defense-related, transporter, and metabolism, all of which have been associated with disease responses in Arabidopsis and rice, suggesting similar response pathways are involved in apple blossoms.

Original languageEnglish (US)
Pages (from-to)4851-4861
Number of pages11
JournalJournal of experimental botany
Volume62
Issue number14
DOIs
StatePublished - Oct 2011

Keywords

  • Apple
  • Malus×domestica
  • fire blight
  • gene expression
  • gene regulation
  • microarray

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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