Expression order of alpha-v and beta-3 integrin subunits in the N-methyl-N-nitrosourea-induced rat mammary tumor model

Robabeh Rezaeipoor; Eric J. Chaney; Amy L. Oldenburg; Stephen A. Boppart

doi:10.1080/07357900802620836

Expression order of alpha-v and beta-3 integrin subunits in the N-methyl-N-nitrosourea-induced rat mammary tumor model

Robabeh Rezaeipoor, Eric J. Chaney, Amy L. Oldenburg, Stephen A. Boppart

Research output: Contribution to journal › Article › peer-review

Abstract

We investigated the developmental time course of molecular expression of αvβ3 subunits in a carcinogen-induced rat mammary tumor model for human ductal carcinoma in situ (DCIS). Tumors during various stages of growth (from <0.1 to >2.0 cm) were analyzed immunohistochemically for the expression of the αvβ3 integrin and its subunits. In general, the expression profiles of these integrin subunits were directly proportional to the size of the tumor. The pattern of immunostaining revealed that anti-αvβ3 monoclonal antibody binds to specific sites of tumor sections, forming isolated stained patches. This isolated patch pattern was found in more developed larger tumors. This could be due to the fact that the integrin molecule might be involved in migration and nesting of tumor cells into specific regions to form DCIS or intraductal carcinoma. Results also showed that the αv subunit expresses earlier than the β3 subunit. These data provide insight into tumor cell biology and developmental characteristics that will guide the future construction and use of targeted contrast and therapeutic agents capable of tracking, imaging, or treating a tumor at the earliest stage of formation.

Original language	English (US)
Pages (from-to)	496-503
Number of pages	8
Journal	Cancer Investigation
Volume	27
Issue number	5
DOIs	https://doi.org/10.1080/07357900802620836
State	Published - Jun 2009

Keywords

Alpha-v beta-3 subunits
Breast cancer
Expression
Integrin
Mammary tumors
Molecular imaging

ASJC Scopus subject areas

Cancer Research
Oncology

Online availability

10.1080/07357900802620836

Library availability

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@article{2987e257b9f84fada44403010758c9ee,

title = "Expression order of alpha-v and beta-3 integrin subunits in the N-methyl-N-nitrosourea-induced rat mammary tumor model",

abstract = "We investigated the developmental time course of molecular expression of αvβ3 subunits in a carcinogen-induced rat mammary tumor model for human ductal carcinoma in situ (DCIS). Tumors during various stages of growth (from <0.1 to >2.0 cm) were analyzed immunohistochemically for the expression of the αvβ3 integrin and its subunits. In general, the expression profiles of these integrin subunits were directly proportional to the size of the tumor. The pattern of immunostaining revealed that anti-αvβ3 monoclonal antibody binds to specific sites of tumor sections, forming isolated stained patches. This isolated patch pattern was found in more developed larger tumors. This could be due to the fact that the integrin molecule might be involved in migration and nesting of tumor cells into specific regions to form DCIS or intraductal carcinoma. Results also showed that the αv subunit expresses earlier than the β3 subunit. These data provide insight into tumor cell biology and developmental characteristics that will guide the future construction and use of targeted contrast and therapeutic agents capable of tracking, imaging, or treating a tumor at the earliest stage of formation.",

keywords = "Alpha-v beta-3 subunits, Breast cancer, Expression, Integrin, Mammary tumors, Molecular imaging",

author = "Robabeh Rezaeipoor and Chaney, {Eric J.} and Oldenburg, {Amy L.} and Boppart, {Stephen A.}",

note = "Funding Information: We acknowledge helpful collaborative interactions with Prof. Samuel Achilefu from the Department of Radiology and Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri. We also wish to thank Dr. Rohit Bhargava and his graduate student Ro-hith K. Reddy from the Department of Bioengineering and the Beckman Institute for Advanced Science and Technology at the University of Illinois at Urbana-Champaign for their assistance in quantification of this data. This work was supported in part by the National Institutes of Health (Roadmap Initiative, NIBIB, 1 R21 EB005321 and 1 R01 EB005221, S.A.B.), and by NCI U54-CA119342-01 to the Siteman Center of Cancer Nanotechnology Excellence, through the University of Illinois Center for Nanoscale Science and Technology. Additional information can be found at http://biophotonics.uiuc.edu.",

year = "2009",

month = jun,

doi = "10.1080/07357900802620836",

language = "English (US)",

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T1 - Expression order of alpha-v and beta-3 integrin subunits in the N-methyl-N-nitrosourea-induced rat mammary tumor model

AU - Rezaeipoor, Robabeh

AU - Chaney, Eric J.

AU - Oldenburg, Amy L.

AU - Boppart, Stephen A.

N1 - Funding Information: We acknowledge helpful collaborative interactions with Prof. Samuel Achilefu from the Department of Radiology and Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri. We also wish to thank Dr. Rohit Bhargava and his graduate student Ro-hith K. Reddy from the Department of Bioengineering and the Beckman Institute for Advanced Science and Technology at the University of Illinois at Urbana-Champaign for their assistance in quantification of this data. This work was supported in part by the National Institutes of Health (Roadmap Initiative, NIBIB, 1 R21 EB005321 and 1 R01 EB005221, S.A.B.), and by NCI U54-CA119342-01 to the Siteman Center of Cancer Nanotechnology Excellence, through the University of Illinois Center for Nanoscale Science and Technology. Additional information can be found at http://biophotonics.uiuc.edu.

PY - 2009/6

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N2 - We investigated the developmental time course of molecular expression of αvβ3 subunits in a carcinogen-induced rat mammary tumor model for human ductal carcinoma in situ (DCIS). Tumors during various stages of growth (from <0.1 to >2.0 cm) were analyzed immunohistochemically for the expression of the αvβ3 integrin and its subunits. In general, the expression profiles of these integrin subunits were directly proportional to the size of the tumor. The pattern of immunostaining revealed that anti-αvβ3 monoclonal antibody binds to specific sites of tumor sections, forming isolated stained patches. This isolated patch pattern was found in more developed larger tumors. This could be due to the fact that the integrin molecule might be involved in migration and nesting of tumor cells into specific regions to form DCIS or intraductal carcinoma. Results also showed that the αv subunit expresses earlier than the β3 subunit. These data provide insight into tumor cell biology and developmental characteristics that will guide the future construction and use of targeted contrast and therapeutic agents capable of tracking, imaging, or treating a tumor at the earliest stage of formation.

AB - We investigated the developmental time course of molecular expression of αvβ3 subunits in a carcinogen-induced rat mammary tumor model for human ductal carcinoma in situ (DCIS). Tumors during various stages of growth (from <0.1 to >2.0 cm) were analyzed immunohistochemically for the expression of the αvβ3 integrin and its subunits. In general, the expression profiles of these integrin subunits were directly proportional to the size of the tumor. The pattern of immunostaining revealed that anti-αvβ3 monoclonal antibody binds to specific sites of tumor sections, forming isolated stained patches. This isolated patch pattern was found in more developed larger tumors. This could be due to the fact that the integrin molecule might be involved in migration and nesting of tumor cells into specific regions to form DCIS or intraductal carcinoma. Results also showed that the αv subunit expresses earlier than the β3 subunit. These data provide insight into tumor cell biology and developmental characteristics that will guide the future construction and use of targeted contrast and therapeutic agents capable of tracking, imaging, or treating a tumor at the earliest stage of formation.

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Expression order of alpha-v and beta-3 integrin subunits in the N-methyl-N-nitrosourea-induced rat mammary tumor model

Abstract

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