Expression of type I and type II interleukin-1 receptors in mouse brain

Patricia Parnet, Simin Amindari, Chiang Wu, Deborah Brunke-Reese, Emmanuelle Goujon, James A. Weyhenmeyer, Robert Dantzer, Keith W. Kelley

Research output: Contribution to journalArticlepeer-review


Although binding sites for IL-1 have been identified in the mouse brain, it is still unknown whether these binding sites correspond to the type I or type II IL-1 receptor. Quantitative autoradiography was used to confirm the presence of specific binding sites for radiolabelled recombinant human IL-1α (125I-HuIL-1α) in the brain of DBA/2 mice. IL-1 binding was highest in the dentate gyrus, consisting of a single class of high affinity binding sites with a Kd of 0.1 nM and a Bmax of 57 fmol/mg protein. A similar Kd of 0.2 nM was obtained using isolated membranes from the whole hippocampus, although the number of binding sites was lower (2 fmol/mg protein). Affinity cross-linking of 125I-Hu-IL-1α to hipocampal membranes revealed the existence of two types of IL-1 receptor proteins, consistent with the sizes of the type I (85 kD) and type II (60 kD) IL-1 receptor. Oligonucleotide probes were then synthesized and used in RT-PCR followed by Southern blotting to show that the whole brain expresses transcripts for both the type I and type II IL-1 receptors. The murine neuroblastoma cell line, C1300, expresses type I rather than type II IL-1 receptor mRNA. The type I receptor protein can be identified by flow cytometry on the membrane of the C1300 neuronal cell line using indirect immunofluorescence with a rat anti-mouse type I IL-1 receptor MoAb. These data show that mouse brain expresses both type I and type II IL-1 receptor mRNA and proteins and offer further support to the idea that type I IL-1 receptors are synthesized and expressed by neurons.

Original languageEnglish (US)
Pages (from-to)63-70
Number of pages8
JournalMolecular Brain Research
Issue number1
StatePublished - Nov 1994


  • Brain
  • Immunodetection
  • Interleukin-1 receptor
  • Neuron
  • Quantitative autoradiography
  • RT-PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience


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