Expression of the tumor necrosis factor locus is not necessary for the cytolytic activity of T lymphocytes

C. V. Jongeneel, S. A. Nedospasov, G. Plaetinck, P. Naquet, J. C. Cerottini

Research output: Contribution to journalArticlepeer-review

Abstract

In order to test whether tumor necrosis factors α (TNF-α) or β (TNF-β, also known as lymphotoxin) are involved in the lysis of target cells by cytolytic T lymphocytes, we probed for the presence of the TNF mRNAs in several quiescent and activated CTL clones. No TNF mRNA could be found in constitutively cytolytic Lyt-2+ clones, and only two out of three clones tested accumulated TNF mRNA after stimulation with phorbol myristate acetate and ionomycin. Of two L3T4+ clones that can be induced to become cytolytic by a combination of antigen and IL-1, only one accumulated TNF-β mRNA in the process. The PC60 rat x mouse T cell hybrid, which becomes cytolytic in response to a combination of IL-1 and IL-2, also failed to accumulate TNF mRNA after stimulation with these agents. Our results strongly suggest that TNF-α or -β are not necessary agents of the cytolytic activity exhibited by antigen-specific T lymphocytes.

Original languageEnglish (US)
Pages (from-to)1916-1922
Number of pages7
JournalJournal of Immunology
Volume140
Issue number6
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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