Expression of the T-cell-specific γ gene is unnecessary in T cells recognizing class II MHC determinants

J. S. Heilig, L. H. Glimcher, David M Kranz, L. K. Clayton, J. L. Greenstein, H. Saito, A. M. Maxam, S. J. Burakoff, H. N. Eisen, S. Tonegawa

Research output: Contribution to journalArticle

Abstract

Subtractive complementary DNA cloning combined with partial protein sequencing has allowed identification of the genes encoding the α and β subunits of T-cell receptors1-7. The subtractive cDNA library prepared from the cytotoxic T lymphocyte (Tc) clone 2C has been found to contain a third type of clone encoding the γ chain5,8,9. The γ gene shares several features with the α and β genes: (1) Assembly from gene segments resembling immunoglobulin V, J and C (respectively variable, joining and constant region) DNA segments10; (2) rearrangement and expression in T cells and not in B cells8; (3) sequences reminiscent of transmembrane and intracytoplasmic regions of integral membrane proteins8; (4) a cysteine residue at the position expected for an interchain disulphide bond. The α and β genes are expressed at equivalent levels in both Tc cells and helper T cells (T H)4,5,9,11. The γ gene, obtained from 2C, has been found to be expressed in all Tc cells studied9. Here we present evidence that strongly suggests that TH cells do not require γ gene expression.

Original languageEnglish (US)
Pages (from-to)68-70
Number of pages3
JournalNature
Volume317
Issue number6032
DOIs
StatePublished - Dec 1 1985
Externally publishedYes

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T-Lymphocytes
Genes
Clone Cells
Immunoglobulin Variable Region
Protein Sequence Analysis
Cytotoxic T-Lymphocytes
Helper-Inducer T-Lymphocytes
Gene Library
Disulfides
Cysteine
Organism Cloning
Complementary DNA
Gene Expression
Membranes
DNA

ASJC Scopus subject areas

  • General

Cite this

Heilig, J. S., Glimcher, L. H., Kranz, D. M., Clayton, L. K., Greenstein, J. L., Saito, H., ... Tonegawa, S. (1985). Expression of the T-cell-specific γ gene is unnecessary in T cells recognizing class II MHC determinants. Nature, 317(6032), 68-70. https://doi.org/10.1038/317068a0

Expression of the T-cell-specific γ gene is unnecessary in T cells recognizing class II MHC determinants. / Heilig, J. S.; Glimcher, L. H.; Kranz, David M; Clayton, L. K.; Greenstein, J. L.; Saito, H.; Maxam, A. M.; Burakoff, S. J.; Eisen, H. N.; Tonegawa, S.

In: Nature, Vol. 317, No. 6032, 01.12.1985, p. 68-70.

Research output: Contribution to journalArticle

Heilig, JS, Glimcher, LH, Kranz, DM, Clayton, LK, Greenstein, JL, Saito, H, Maxam, AM, Burakoff, SJ, Eisen, HN & Tonegawa, S 1985, 'Expression of the T-cell-specific γ gene is unnecessary in T cells recognizing class II MHC determinants', Nature, vol. 317, no. 6032, pp. 68-70. https://doi.org/10.1038/317068a0
Heilig, J. S. ; Glimcher, L. H. ; Kranz, David M ; Clayton, L. K. ; Greenstein, J. L. ; Saito, H. ; Maxam, A. M. ; Burakoff, S. J. ; Eisen, H. N. ; Tonegawa, S. / Expression of the T-cell-specific γ gene is unnecessary in T cells recognizing class II MHC determinants. In: Nature. 1985 ; Vol. 317, No. 6032. pp. 68-70.
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