Expression of the nucleocapsid protein of Porcine Reproductive and Respiratory Syndrome Virus in soybean seed yields an immunogenic antigenic protein

Sornkanok Vimolmangkang, Ksenija Gasic, Ruth Soria-Guerra, Sergio Rosales-Mendoza, Leticia Moreno-Fierros, Schuyler S. Korban

Research output: Contribution to journalArticle

Abstract

Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is a serious disease of swine and contributes to severe worldwide economic losses in swine production. Current vaccines against PRRS rely on the use of an attenuated-live virus; however, these are unreliable. Thus, alternative effective vaccines against PRRS are needed. Plant-based subunit vaccines offer viable, safe, and environmentally friendly alternatives to conventional vaccines. In this study, efforts have been undertaken to develop a soybean-based vaccine against PRRSV. A construct carrying a synthesized PRRSV-ORF7 antigen, nucleocapsid N protein of PRRSV, has been introduced into soybean, Glycine max (L.) Merrill. cvs. Jack and Kunitz, using Agrobacterium-mediated transformation. Transgenic plants carrying the sORF7 transgene have been successfully generated. Molecular analyses of T 0 plants confirmed integration of the transgene and transcription of the PRRSV-ORF7. Presence of a 15-kDa protein in seeds of T 1 transgenic lines was confirmed by Western blot analysis using PRRSV-ORF7 antisera. The amount of the antigenic protein accumulating in seeds of these transgenic lines was up to 0. 65% of the total soluble protein (TSP). A significant induction of a specific immune response, both humoral and mucosal, against PRRSV-ORF7 was observed following intragastric immunization of BALB/c female mice with transgenic soybean seeds. These findings provide a 'proof of concept', and serve as a critical step in the development of a subunit plant-based vaccine against PRRS.

Original languageEnglish (US)
Pages (from-to)513-522
Number of pages10
JournalPlanta
Volume235
Issue number3
DOIs
StatePublished - Mar 2012

Keywords

  • Immunogenicity
  • PRRS viral subunit
  • Plant-based vaccine
  • Transgenic soybean

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of 'Expression of the nucleocapsid protein of Porcine Reproductive and Respiratory Syndrome Virus in soybean seed yields an immunogenic antigenic protein'. Together they form a unique fingerprint.

  • Cite this