Expression of soybean lectin gene deletions in tobacco

Jon T. Lindstrom, Lila O Vodkin, Roy W. Harding, Robert M. Goeken

Research output: Contribution to journalArticle

Abstract

A series of constructs containing the developmentally regulated soybean lectin gene (Le1) were used to transform tobacco plants in order to assess developmental and quantitative regulation conferred by flanking sequences. The largest of the lectin constructs contained approximately 3,00 base pairs (bp) of Le1 5′ flanking region and 1,500 bp of the 3′ flanking region. The smallest construct contained no 5′ flanking region and 194 bp of the 3′ flanking region. ELISA assays of lectin in individual tobacco seeds and Southern blot analyses confirmed that most constructs were inherited as unique insertion events. Maximal expression of Le1 required more than 338 bp of 5′ sequence, indicating that far upstream factors are involved in quantitative control of lectin expression. Lectin expression declined more than 80% between deletions with 1,700 versus 338 bp of 5′ flanking sequence. In contrast, developmental control of lectin expression was maintained by Le1 inserts with only 190 bp of 5′ sequence. The lectin promoter offers a potential means to target high levels of gene expression to the developing seeds of soybean or other dicotyledonous plants.

Original languageEnglish (US)
Pages (from-to)160-167
Number of pages8
JournalDevelopmental Genetics
Volume11
Issue number2
DOIs
StatePublished - 1990

Fingerprint

Gene Deletion
Lectins
Base Pairing
Tobacco
5' Flanking Region
3' Flanking Region
Seeds
Southern Blotting
Soybeans
soybean lectin
Enzyme-Linked Immunosorbent Assay
Gene Expression
Genes

Keywords

  • Flanking sequences
  • gene expression
  • insertion events

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Expression of soybean lectin gene deletions in tobacco. / Lindstrom, Jon T.; Vodkin, Lila O; Harding, Roy W.; Goeken, Robert M.

In: Developmental Genetics, Vol. 11, No. 2, 1990, p. 160-167.

Research output: Contribution to journalArticle

Lindstrom, Jon T. ; Vodkin, Lila O ; Harding, Roy W. ; Goeken, Robert M. / Expression of soybean lectin gene deletions in tobacco. In: Developmental Genetics. 1990 ; Vol. 11, No. 2. pp. 160-167.
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