Expression of NGF, BDNF and their receptors in subcutaneous adipose tissue of lactating cows

Monica Colitti, Juan J. Loor, Bruno Stefanon

Research output: Contribution to journalArticlepeer-review

Abstract

Currently, there are no reports of neurotrophins in adipose tissue of cows. The distribution of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and their high-affinity tyrosine kinase receptors TrkA and TrkB, was investigated by immunohistochemical method in the subcutaneous adipose tissue of cow at mid-lactation. Results revealed the localization of NGF and BDNF along the plasma membrane and cytoplasm of adipocytes. Neurotrophin receptors TrkA and TrkB showed moderate and strong positive staining in adipocytes, respectively. The expression of NGF, BDNF, TRKB - but not of TRKA - was also confirmed at transcriptional level by RT-PCR analyses.Considering the involvement of BDNF on fat metabolism and of NGF on activation of the sympathetic response in human and rodents, these neurotrophins could be related to lipogenesis and lipolysis occurring during lactation in cows. The local production of these neurotrophins supports their potential paracrine function for the regulation of adipocyte activity and deserve further investigations.

Original languageEnglish (US)
Pages (from-to)196-199
Number of pages4
JournalResearch in Veterinary Science
Volume102
DOIs
StatePublished - Oct 1 2015

Keywords

  • Adipose tissue
  • BDNF
  • Cow
  • Expression
  • NGF
  • Receptors

ASJC Scopus subject areas

  • veterinary(all)

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