Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain

Giovanni Lugli, Vetle I. Torvik, John Larson, Neil R. Smalheiser

Research output: Contribution to journalArticlepeer-review


We have characterized the expression of microRNAs and selected microRNA precursors within several synaptic fractions of adult mouse forebrain, including synaptoneurosomes, synap - tosomes and isolated post-synaptic densities (PSDs), using methods of microRNA microarray, real time qRT-PCR, Northern blotting and immunopurification using anti-PSD95 antibody. The majority of brain microRNAs (especially microRNAs known to be expressed in pyramidal neurons) are detectably expressed in synaptic fractions, and a subset of microRNAs is significantly enriched in synaptic fractions relative to total fore - brain homogenate. MicroRNA precursors are also detectable in synaptic fractions at levels that are comparable to whole tissue. Whereas mature microRNAs are predominantly associated with soluble components of the synaptic fractions, microRNA precursors are predominantly associated with PSDs. For seven microRNAs examined, there was a significant correlation between the relative synaptic enrichment of the precursor and the relative synaptic enrichment of the corresponding mature microRNA. These findings support the proposal that microRNAs are formed, at least in part, via processing of microRNA precursors locally within dendritic spines. Dicer is expressed in PSDs but is enzymatically inactive until conditions that activate calpain cause its liberation; thus, we propose that synaptic stimulation may lead to local processing of microRNA precursors in proximity to the synapse.

Original languageEnglish (US)
Pages (from-to)650-661
Number of pages12
JournalJournal of Neurochemistry
Issue number2
StatePublished - Jul 2008
Externally publishedYes


  • Dicer
  • Fragile X mental retardation protein
  • Micro - RNA
  • Post-synaptic density
  • Synaptic plasticity
  • microRNA precursors

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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