Expression of Lactococcus lactis NADH oxidase increases 2,3-butanediol production in Pdc-deficient Saccharomyces cerevisiae

Jin Woo Kim, Seung Oh Seo, Guo Chang Zhang, Yong Su Jin, Jin Ho Seo

Research output: Contribution to journalArticle

Abstract

To minimize glycerol production during 2,3-BD fermentation by the engineered Saccharomyces cerevisiae, the Lactococcus lactis water-forming NADH oxidase gene (noxE) was expressed at five different levels. The expression of NADH oxidase substantially decreased the intracellular NADH/NAD+ ratio. The S. cerevisiae BD5-T2nox strain expressing noxE produced 2,3-BD with yield of 0.359 g 2,3-BD/g glucose and glycerol with 0.069 g glycerol/g glucose, which are 23.8% higher and 65.3% lower than those of the isogenic strain without noxE. These results demonstrate that the carbon flux could be redirected from glycerol to 2,3-BD through alteration of the NADH/NAD+ ratio by the expression of NADH oxidase.

Original languageEnglish (US)
Pages (from-to)512-519
Number of pages8
JournalBioresource Technology
Volume191
DOIs
StatePublished - Jun 8 2015

Keywords

  • 2,3-Butanediol
  • Cofactor engineering
  • Glycerol
  • NADH oxidase
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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