Expression of genomic functional estrogen receptor 1 in mouse sertoli cells

Jing Lin, Jia Zhu, Xian Li, Shengqiang Li, Zijian Lan, Jay Ko, Zhenmin Lei

Research output: Contribution to journalArticle

Abstract

There is no consensus whether Sertoli cells express estrogen receptor 1 (Esr1). Reverse transcription-polymerase chain reaction, Western blot, and immunofluorescence demonstrated that mouse Sertoli cell lines, TM4, MSC-1, and 15P-1, and purified primary mouse Sertoli cells (PSCs) contained Esr1 messenger RNA and proteins. Incubation of Sertoli cells with 17β-estradiol (E2) or ESR1 agonist stimulated the expression of an estrogen responsive gene Greb1, which was prevented by ESR inhibitor or ESR1 antagonist. Overexpression of Esr1 in MSC-1 enhanced E2-induced Greb1 expression, while knockdown of Esr1 by small interfering RNA in TM4 attenuated the response. Furthermore, E2-induced Greb1 expression was abolished in the PSCs isolated from Amh-Cre/Esr1-floxed mice in which Esr1 in Sertoli cells were selectively deleted. Chromatin immunoprecipitation assays indicated that E2-induced Greb1 expression in Sertoli cells was mediated by binding of ESR1 to estrogen responsive elements. In summary, ligand-dependent nuclear ESR1 was present in mouse Sertoli cells and mediates a classical genomic action of estrogens.

Original languageEnglish (US)
Pages (from-to)1411-1422
Number of pages12
JournalReproductive Sciences
Volume21
Issue number11
DOIs
StatePublished - Nov 12 2014

Fingerprint

Estrogen Receptor alpha
Sertoli Cells
Estrogens
Chromatin Immunoprecipitation
Small Interfering RNA
Reverse Transcription
Fluorescent Antibody Technique
Estradiol
Western Blotting
Ligands
Cell Line
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Esr1
  • Estrogen
  • Gene regulation
  • Mouse
  • Sertoli cells
  • Testis

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Expression of genomic functional estrogen receptor 1 in mouse sertoli cells. / Lin, Jing; Zhu, Jia; Li, Xian; Li, Shengqiang; Lan, Zijian; Ko, Jay; Lei, Zhenmin.

In: Reproductive Sciences, Vol. 21, No. 11, 12.11.2014, p. 1411-1422.

Research output: Contribution to journalArticle

Lin, Jing ; Zhu, Jia ; Li, Xian ; Li, Shengqiang ; Lan, Zijian ; Ko, Jay ; Lei, Zhenmin. / Expression of genomic functional estrogen receptor 1 in mouse sertoli cells. In: Reproductive Sciences. 2014 ; Vol. 21, No. 11. pp. 1411-1422.
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AU - Lei, Zhenmin

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