Expression of genomic functional estrogen receptor 1 in mouse sertoli cells

Jing Lin, Jia Zhu, Xian Li, Shengqiang Li, Zijian Lan, Jay Ko, Zhenmin Lei

Research output: Contribution to journalArticlepeer-review


There is no consensus whether Sertoli cells express estrogen receptor 1 (Esr1). Reverse transcription-polymerase chain reaction, Western blot, and immunofluorescence demonstrated that mouse Sertoli cell lines, TM4, MSC-1, and 15P-1, and purified primary mouse Sertoli cells (PSCs) contained Esr1 messenger RNA and proteins. Incubation of Sertoli cells with 17β-estradiol (E2) or ESR1 agonist stimulated the expression of an estrogen responsive gene Greb1, which was prevented by ESR inhibitor or ESR1 antagonist. Overexpression of Esr1 in MSC-1 enhanced E2-induced Greb1 expression, while knockdown of Esr1 by small interfering RNA in TM4 attenuated the response. Furthermore, E2-induced Greb1 expression was abolished in the PSCs isolated from Amh-Cre/Esr1-floxed mice in which Esr1 in Sertoli cells were selectively deleted. Chromatin immunoprecipitation assays indicated that E2-induced Greb1 expression in Sertoli cells was mediated by binding of ESR1 to estrogen responsive elements. In summary, ligand-dependent nuclear ESR1 was present in mouse Sertoli cells and mediates a classical genomic action of estrogens.

Original languageEnglish (US)
Pages (from-to)1411-1422
Number of pages12
JournalReproductive Sciences
Issue number11
StatePublished - Nov 12 2014


  • Esr1
  • Estrogen
  • Gene regulation
  • Mouse
  • Sertoli cells
  • Testis

ASJC Scopus subject areas

  • Obstetrics and Gynecology


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