Expression of fragile X mental retardation protein within the vocal control system of developing and adult male zebra finches

C. Winograd, D. Clayton, S. Ceman

Research output: Contribution to journalArticle

Abstract

Individuals with fragile X syndrome (FXS) are cognitively impaired and have marked speech delays and deficits. Our goal was to characterize expression of fragile X mental retardation protein (FMRP), encoded by Fmr1 fragile X mental retardation 1 gene or transcript (FMR1), in an animal model that learns to vocalize, namely the zebra finch Taeniopygia guttata (Tgu). We cloned and sequenced the zebra finch ortholog of FMR1 (TguFmr1) and developed an antibody that recognizes TguFmrp specifically. TguFmrp has structural features similar to its human ortholog FMRP. Because FXS patients exhibit sensorimotor deficits, we examined TguFmrp expression prior to, during, and after sensorimotor song learning in zebra finches. We found that TguFmrp is expressed throughout the brain and in four major song nuclei of the male zebra finch brain, primarily in neurons. Additionally, prior to sensorimotor learning, we observed elevated TguFmrp expression in the robust nucleus of the arcopallium (RA) of post-hatch day 30 males, compared with the surrounding telencephalon, suggesting a preparation for this stage of song learning. Finally, we observed variable TguFmrp expression in the RA of adolescent and adult males: in some males it was elevated and in others it was comparable to the surrounding telencephalon. In summary, we have characterized the zebra finch ortholog of FMRP and found elevated levels in the premotor nucleus RA at a key developmental stage for vocal learning.

Original languageEnglish (US)
Pages (from-to)132-142
Number of pages11
JournalNeuroscience
Volume157
Issue number1
DOIs
StatePublished - Nov 11 2008

Fingerprint

Fragile X Mental Retardation Protein
Finches
Equidae
Music
Learning
Fragile X Syndrome
Telencephalon
Language Development Disorders
Brain
Intellectual Disability
Animal Models
Neurons
Antibodies
Genes

Keywords

  • FMRP
  • brain development
  • songbird
  • vocal learning

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of fragile X mental retardation protein within the vocal control system of developing and adult male zebra finches. / Winograd, C.; Clayton, D.; Ceman, S.

In: Neuroscience, Vol. 157, No. 1, 11.11.2008, p. 132-142.

Research output: Contribution to journalArticle

@article{9e5849d44a454e20b5816e7b481d46cb,
title = "Expression of fragile X mental retardation protein within the vocal control system of developing and adult male zebra finches",
abstract = "Individuals with fragile X syndrome (FXS) are cognitively impaired and have marked speech delays and deficits. Our goal was to characterize expression of fragile X mental retardation protein (FMRP), encoded by Fmr1 fragile X mental retardation 1 gene or transcript (FMR1), in an animal model that learns to vocalize, namely the zebra finch Taeniopygia guttata (Tgu). We cloned and sequenced the zebra finch ortholog of FMR1 (TguFmr1) and developed an antibody that recognizes TguFmrp specifically. TguFmrp has structural features similar to its human ortholog FMRP. Because FXS patients exhibit sensorimotor deficits, we examined TguFmrp expression prior to, during, and after sensorimotor song learning in zebra finches. We found that TguFmrp is expressed throughout the brain and in four major song nuclei of the male zebra finch brain, primarily in neurons. Additionally, prior to sensorimotor learning, we observed elevated TguFmrp expression in the robust nucleus of the arcopallium (RA) of post-hatch day 30 males, compared with the surrounding telencephalon, suggesting a preparation for this stage of song learning. Finally, we observed variable TguFmrp expression in the RA of adolescent and adult males: in some males it was elevated and in others it was comparable to the surrounding telencephalon. In summary, we have characterized the zebra finch ortholog of FMRP and found elevated levels in the premotor nucleus RA at a key developmental stage for vocal learning.",
keywords = "FMRP, brain development, songbird, vocal learning",
author = "C. Winograd and D. Clayton and S. Ceman",
year = "2008",
month = "11",
day = "11",
doi = "10.1016/j.neuroscience.2008.09.005",
language = "English (US)",
volume = "157",
pages = "132--142",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Expression of fragile X mental retardation protein within the vocal control system of developing and adult male zebra finches

AU - Winograd, C.

AU - Clayton, D.

AU - Ceman, S.

PY - 2008/11/11

Y1 - 2008/11/11

N2 - Individuals with fragile X syndrome (FXS) are cognitively impaired and have marked speech delays and deficits. Our goal was to characterize expression of fragile X mental retardation protein (FMRP), encoded by Fmr1 fragile X mental retardation 1 gene or transcript (FMR1), in an animal model that learns to vocalize, namely the zebra finch Taeniopygia guttata (Tgu). We cloned and sequenced the zebra finch ortholog of FMR1 (TguFmr1) and developed an antibody that recognizes TguFmrp specifically. TguFmrp has structural features similar to its human ortholog FMRP. Because FXS patients exhibit sensorimotor deficits, we examined TguFmrp expression prior to, during, and after sensorimotor song learning in zebra finches. We found that TguFmrp is expressed throughout the brain and in four major song nuclei of the male zebra finch brain, primarily in neurons. Additionally, prior to sensorimotor learning, we observed elevated TguFmrp expression in the robust nucleus of the arcopallium (RA) of post-hatch day 30 males, compared with the surrounding telencephalon, suggesting a preparation for this stage of song learning. Finally, we observed variable TguFmrp expression in the RA of adolescent and adult males: in some males it was elevated and in others it was comparable to the surrounding telencephalon. In summary, we have characterized the zebra finch ortholog of FMRP and found elevated levels in the premotor nucleus RA at a key developmental stage for vocal learning.

AB - Individuals with fragile X syndrome (FXS) are cognitively impaired and have marked speech delays and deficits. Our goal was to characterize expression of fragile X mental retardation protein (FMRP), encoded by Fmr1 fragile X mental retardation 1 gene or transcript (FMR1), in an animal model that learns to vocalize, namely the zebra finch Taeniopygia guttata (Tgu). We cloned and sequenced the zebra finch ortholog of FMR1 (TguFmr1) and developed an antibody that recognizes TguFmrp specifically. TguFmrp has structural features similar to its human ortholog FMRP. Because FXS patients exhibit sensorimotor deficits, we examined TguFmrp expression prior to, during, and after sensorimotor song learning in zebra finches. We found that TguFmrp is expressed throughout the brain and in four major song nuclei of the male zebra finch brain, primarily in neurons. Additionally, prior to sensorimotor learning, we observed elevated TguFmrp expression in the robust nucleus of the arcopallium (RA) of post-hatch day 30 males, compared with the surrounding telencephalon, suggesting a preparation for this stage of song learning. Finally, we observed variable TguFmrp expression in the RA of adolescent and adult males: in some males it was elevated and in others it was comparable to the surrounding telencephalon. In summary, we have characterized the zebra finch ortholog of FMRP and found elevated levels in the premotor nucleus RA at a key developmental stage for vocal learning.

KW - FMRP

KW - brain development

KW - songbird

KW - vocal learning

UR - http://www.scopus.com/inward/record.url?scp=54549084263&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54549084263&partnerID=8YFLogxK

U2 - 10.1016/j.neuroscience.2008.09.005

DO - 10.1016/j.neuroscience.2008.09.005

M3 - Article

C2 - 18835331

AN - SCOPUS:54549084263

VL - 157

SP - 132

EP - 142

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 1

ER -