The activity of Escherichia coli pyruvate oxidase (PoxB) was shown to be growth‐phase dependent; the enzyme activity reaches a maximum at early stationary phase. We report that PoxB activity is dependent on a functional rpoS(katF) gene which encodes a σ factor required to transcribe a number of stationary‐phase‐induced genes. PoxB activity as well as the β‐galactosidase encoded by a poxB::lacZ protein fusion was completely abolished in a strain containing a defective rpoS gene. Northern and primer extension analyses showed that poxB expression was regulated at the transcriptional level and was transcribed from a single promoter; the 5′ end of the mRNA being located 27 bp upstream of the translational initiation codon of poxB. The poxB gene was expressed at decreased levels under anaerobiosis; however, the anaerobic regulatory genes arcA, arcB or fnr were not involved in anaerobic poxB gene expression. Expression of the rpoS(katF) gene has been reported to be affected by acetate, the product of PoxB reaction. However, we found that poxB null mutations had no effect on rpoS(katF) expression. Inactivation of two genes involved In acetate metabolism, ackA and pta, had no effect on either poxB or rpoS(katF) expression.
|Original language||English (US)|
|Number of pages||10|
|State||Published - Mar 1994|
ASJC Scopus subject areas
- Molecular Biology