Expression of cytochromes P450 4F4 and 4F5 in infection and injury models of inflammation

X. Cui; A. Kalsotra; A. M. Robida; D. Matzilevich; A. N. Moore; C. L. Boehme; E. T. Morgan; P. K. Dash; H. W. Strobel

doi:10.1016/S0304-4165(02)00491-9

Expression of cytochromes P450 4F4 and 4F5 in infection and injury models of inflammation

X. Cui, A. Kalsotra, A. M. Robida, D. Matzilevich, A. N. Moore, C. L. Boehme, E. T. Morgan, P. K. Dash, H. W. Strobel

Research output: Contribution to journal › Article › peer-review

Abstract

Lipopolysaccharide (LPS) treatment of rats suppresses CYP 4F4 and 4F5 expression by 50 and 40%, respectively, in a direct fashion occurring in the liver. This contention is borne out by essentially parallel dose-dependent changes observed upon treatment of rat hepatocyte cultures with LPS. An alternate avenue of triggering the inflammatory cascade is traumatic brain injury by controlled cortical impact. Such injury brings about a dramatic change in the expression of CYP 4F4 and 4F5 mRNA which reaches its greatest effect 24 h after impact compared with sham-operated but uninjured controls. At time points after 24 h the expression of both isoforms increases dramatically reaching highest levels at 2 weeks post-injury. These changes in mRNA expression are mirrored by changes in protein expression. The results are consistent with the notion that immediately after injury concentrations of leukotriene and prostaglandin mediators are elevated by decreased CYP 4F concentrations. As time after injury increases those conditions reverse. Increased CYP 4F expression leads to diminished concentrations of leukotriene and prostaglandin mediators and then to recovery and repair.

Original language	English (US)
Pages (from-to)	325-331
Number of pages	7
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	1619
Issue number	3
DOIs	https://doi.org/10.1016/S0304-4165(02)00491-9
State	Published - Feb 17 2003
Externally published	Yes

Keywords

Brain cytochrome P450 distribution
CYP 4F subfamily expression
CYP 4F subfamily suppression by inflammation
Inflammation and P450 expression
Lipopolysaccharide and CYP 4F subfamily expression
Regulation of CYP 4F expression

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

Online availability

10.1016/S0304-4165(02)00491-9

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title = "Expression of cytochromes P450 4F4 and 4F5 in infection and injury models of inflammation",

abstract = "Lipopolysaccharide (LPS) treatment of rats suppresses CYP 4F4 and 4F5 expression by 50 and 40%, respectively, in a direct fashion occurring in the liver. This contention is borne out by essentially parallel dose-dependent changes observed upon treatment of rat hepatocyte cultures with LPS. An alternate avenue of triggering the inflammatory cascade is traumatic brain injury by controlled cortical impact. Such injury brings about a dramatic change in the expression of CYP 4F4 and 4F5 mRNA which reaches its greatest effect 24 h after impact compared with sham-operated but uninjured controls. At time points after 24 h the expression of both isoforms increases dramatically reaching highest levels at 2 weeks post-injury. These changes in mRNA expression are mirrored by changes in protein expression. The results are consistent with the notion that immediately after injury concentrations of leukotriene and prostaglandin mediators are elevated by decreased CYP 4F concentrations. As time after injury increases those conditions reverse. Increased CYP 4F expression leads to diminished concentrations of leukotriene and prostaglandin mediators and then to recovery and repair.",

keywords = "Brain cytochrome P450 distribution, CYP 4F subfamily expression, CYP 4F subfamily suppression by inflammation, Inflammation and P450 expression, Lipopolysaccharide and CYP 4F subfamily expression, Regulation of CYP 4F expression",

author = "X. Cui and A. Kalsotra and Robida, {A. M.} and D. Matzilevich and Moore, {A. N.} and Boehme, {C. L.} and Morgan, {E. T.} and Dash, {P. K.} and Strobel, {H. W.}",

note = "Funding Information: This work was supported by Grant MH 58297 (HWS), Grant GM 46897 (ETM) from the National Institute of Mental Health and General Medical Sciences, DHHW and by U.S. Army Grant DAMD-17-98-1-8002 Disaster Relief and Emergency Medical Services. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2003",

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doi = "10.1016/S0304-4165(02)00491-9",

language = "English (US)",

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T1 - Expression of cytochromes P450 4F4 and 4F5 in infection and injury models of inflammation

AU - Cui, X.

AU - Kalsotra, A.

AU - Robida, A. M.

AU - Matzilevich, D.

AU - Moore, A. N.

AU - Boehme, C. L.

AU - Morgan, E. T.

AU - Dash, P. K.

AU - Strobel, H. W.

N1 - Funding Information: This work was supported by Grant MH 58297 (HWS), Grant GM 46897 (ETM) from the National Institute of Mental Health and General Medical Sciences, DHHW and by U.S. Army Grant DAMD-17-98-1-8002 Disaster Relief and Emergency Medical Services. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2003/2/17

Y1 - 2003/2/17

N2 - Lipopolysaccharide (LPS) treatment of rats suppresses CYP 4F4 and 4F5 expression by 50 and 40%, respectively, in a direct fashion occurring in the liver. This contention is borne out by essentially parallel dose-dependent changes observed upon treatment of rat hepatocyte cultures with LPS. An alternate avenue of triggering the inflammatory cascade is traumatic brain injury by controlled cortical impact. Such injury brings about a dramatic change in the expression of CYP 4F4 and 4F5 mRNA which reaches its greatest effect 24 h after impact compared with sham-operated but uninjured controls. At time points after 24 h the expression of both isoforms increases dramatically reaching highest levels at 2 weeks post-injury. These changes in mRNA expression are mirrored by changes in protein expression. The results are consistent with the notion that immediately after injury concentrations of leukotriene and prostaglandin mediators are elevated by decreased CYP 4F concentrations. As time after injury increases those conditions reverse. Increased CYP 4F expression leads to diminished concentrations of leukotriene and prostaglandin mediators and then to recovery and repair.

AB - Lipopolysaccharide (LPS) treatment of rats suppresses CYP 4F4 and 4F5 expression by 50 and 40%, respectively, in a direct fashion occurring in the liver. This contention is borne out by essentially parallel dose-dependent changes observed upon treatment of rat hepatocyte cultures with LPS. An alternate avenue of triggering the inflammatory cascade is traumatic brain injury by controlled cortical impact. Such injury brings about a dramatic change in the expression of CYP 4F4 and 4F5 mRNA which reaches its greatest effect 24 h after impact compared with sham-operated but uninjured controls. At time points after 24 h the expression of both isoforms increases dramatically reaching highest levels at 2 weeks post-injury. These changes in mRNA expression are mirrored by changes in protein expression. The results are consistent with the notion that immediately after injury concentrations of leukotriene and prostaglandin mediators are elevated by decreased CYP 4F concentrations. As time after injury increases those conditions reverse. Increased CYP 4F expression leads to diminished concentrations of leukotriene and prostaglandin mediators and then to recovery and repair.

KW - Brain cytochrome P450 distribution

KW - CYP 4F subfamily expression

KW - CYP 4F subfamily suppression by inflammation

KW - Inflammation and P450 expression

KW - Lipopolysaccharide and CYP 4F subfamily expression

KW - Regulation of CYP 4F expression

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ER -

Expression of cytochromes P450 4F4 and 4F5 in infection and injury models of inflammation

Abstract

Keywords

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