TY - JOUR
T1 - Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions
AU - Köhler, Iris H.
AU - Ruiz-Vera, Ursula M.
AU - VanLoocke, Andy
AU - Thomey, Michell L.
AU - Clemente, Tom
AU - Long, Stephen P.
AU - Ort, Donald R.
AU - Bernacchi, Carl J.
N1 - The authors would like to thank David Drag, the SoyFACE technical staff (Brad Dalsing, Kannan Puthuval, Chris Montes, Chad Lantz), and undergraduate students for help with the field experiment. The Raines Lab (Christine Raines, Andrew Simkin, Patricia Lopez, Stuart Fisk, Kenny Brown) is thanked for help with western blotting. This work was supported in part by an appointment to the Agricultural Research Service (ARS) Postdoctoral Research Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the US Department of Energy and the US Department of Agriculture. ORISE is managed by ORAU under DOE contract number DE-AC05-06OR23100. This material is based upon work that is supported by the National Institute of Food and Agriculture, US Department of Agriculture, under award number 2014-67013-21783. All opinions expressed in this paper are the authors' and do not necessarily reflect the policies and views of USDA, ARS, DOE, or ORAU/ORISE.
PY - 2017
Y1 - 2017
N2 - Predictions suggest that current crop production needs to double by 2050 to meet global food and energy demands. Based on theory and experimental studies, overexpression of the photosynthetic enzyme sedoheptulose-1,7-bisphosphatase (SBPase) is expected to enhance C3 crop photosynthesis and yields. Here we test how expression of the cyanobacterial, bifunctional fructose-1,6/sedoheptulose-1,7-bisphosphatase (FBP/SBPase) affects carbon assimilation and seed yield (SY) in a major crop (soybean, Glycine max). For three growing seasons, wild-type (WT) and FBP/SBPase-expressing (FS) plants were grown in the field under ambient (400 mol mol1) and elevated (600 mol mol1) CO2 concentrations [CO2] and under ambient and elevated temperatures (+2.7 °C during daytime, +3.4 °C at night) at the SoyFACE research site. Across treatments, FS plants had significantly higher carbon assimilation (4-14%), Vc,max (5-8%), and Jmax (4-8%). Under ambient [CO2], elevated temperature led to significant reductions of SY of both genotypes by 19-31%. However, under elevated [CO2] and elevated temperature, FS plants maintained SY levels, while the WT showed significant reductions between 11% and 22% compared with plants under elevated [CO2] alone. These results show that the manipulation of the photosynthetic carbon reduction cycle can mitigate the effects of future high CO2 and high temperature environments on soybean yield.
AB - Predictions suggest that current crop production needs to double by 2050 to meet global food and energy demands. Based on theory and experimental studies, overexpression of the photosynthetic enzyme sedoheptulose-1,7-bisphosphatase (SBPase) is expected to enhance C3 crop photosynthesis and yields. Here we test how expression of the cyanobacterial, bifunctional fructose-1,6/sedoheptulose-1,7-bisphosphatase (FBP/SBPase) affects carbon assimilation and seed yield (SY) in a major crop (soybean, Glycine max). For three growing seasons, wild-type (WT) and FBP/SBPase-expressing (FS) plants were grown in the field under ambient (400 mol mol1) and elevated (600 mol mol1) CO2 concentrations [CO2] and under ambient and elevated temperatures (+2.7 °C during daytime, +3.4 °C at night) at the SoyFACE research site. Across treatments, FS plants had significantly higher carbon assimilation (4-14%), Vc,max (5-8%), and Jmax (4-8%). Under ambient [CO2], elevated temperature led to significant reductions of SY of both genotypes by 19-31%. However, under elevated [CO2] and elevated temperature, FS plants maintained SY levels, while the WT showed significant reductions between 11% and 22% compared with plants under elevated [CO2] alone. These results show that the manipulation of the photosynthetic carbon reduction cycle can mitigate the effects of future high CO2 and high temperature environments on soybean yield.
KW - Elevated CO
KW - Elevated temperature
KW - Free air CO enrichment
KW - Glycine max
KW - Sedoheptulose-1,7-bisphosphatase
KW - Soy-T-FACE
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U2 - 10.1093/jxb/erw435
DO - 10.1093/jxb/erw435
M3 - Article
C2 - 28204603
AN - SCOPUS:85016217589
SN - 0022-0957
VL - 68
SP - 715
EP - 726
JO - Journal of experimental botany
JF - Journal of experimental botany
IS - 3
ER -