Expression of an intracisternal A-particle-like element in rat ovary

Katherine Michelle Graham, Chemyong Ko, Kyung Soo Park, Kevin Sarge, Ok Kyong Park-Sarge

Research output: Contribution to journalArticlepeer-review


We have isolated a rat intracisternal-A particle element (IAP)-like element (IAP-LE) from ovarian granulosa cells that appears to be identical to the rat EST clone AA964260. The compiled cDNA sequences contain several putative in-frame translation initiation codons with the largest capable of encoding a 365 amino acid protein with a reverse transcriptase domain in the N-terminus as well as a bipartite nuclear localization signal sequence in the middle. Northern blotting shows a major ~7 Kb transcript and a minor ~5 Kb transcript that are abundantly expressed in the ovary. In situ hybridization histochemistry using ovaries from gonadotropin-treated immature rats and regularly cycling adult rats show that this transcript is predominantly localized to granulosa cells of all healthy follicles, including primary follicles, and to newly-formed and healthy corpora lutea. This cell-specific expression pattern of the IAP-LE gene is distinct from those of the several known retroviral elements, suggesting the potentially novel functional importance of the IAP-LE gene. Taken together, our results demonstrate abundant and cell-specific expression of a novel IAP-LE in rat granulosa cells. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)48-57
Number of pages10
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Nov 11 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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