TY - JOUR
T1 - Expression of an intracisternal A-particle-like element in rat ovary
AU - Graham, Katherine Michelle
AU - Ko, Chemyong
AU - Park, Kyung Soo
AU - Sarge, Kevin
AU - Park-Sarge, Ok Kyong
N1 - Funding Information:
We thank Ms. Lisa Savage for help with in situ hybridization and Dr. Thomas Curry, Jr., for help with follicular morphology. This work was supported by NIH Grants HD30719 and HD36879 to O.K.P.S. O.K.P.S. is a recipient of a NIH Research Career Development Award, HD01135.
PY - 2000/11/11
Y1 - 2000/11/11
N2 - We have isolated a rat intracisternal-A particle element (IAP)-like element (IAP-LE) from ovarian granulosa cells that appears to be identical to the rat EST clone AA964260. The compiled cDNA sequences contain several putative in-frame translation initiation codons with the largest capable of encoding a 365 amino acid protein with a reverse transcriptase domain in the N-terminus as well as a bipartite nuclear localization signal sequence in the middle. Northern blotting shows a major ~7 Kb transcript and a minor ~5 Kb transcript that are abundantly expressed in the ovary. In situ hybridization histochemistry using ovaries from gonadotropin-treated immature rats and regularly cycling adult rats show that this transcript is predominantly localized to granulosa cells of all healthy follicles, including primary follicles, and to newly-formed and healthy corpora lutea. This cell-specific expression pattern of the IAP-LE gene is distinct from those of the several known retroviral elements, suggesting the potentially novel functional importance of the IAP-LE gene. Taken together, our results demonstrate abundant and cell-specific expression of a novel IAP-LE in rat granulosa cells. (C) 2000 Academic Press.
AB - We have isolated a rat intracisternal-A particle element (IAP)-like element (IAP-LE) from ovarian granulosa cells that appears to be identical to the rat EST clone AA964260. The compiled cDNA sequences contain several putative in-frame translation initiation codons with the largest capable of encoding a 365 amino acid protein with a reverse transcriptase domain in the N-terminus as well as a bipartite nuclear localization signal sequence in the middle. Northern blotting shows a major ~7 Kb transcript and a minor ~5 Kb transcript that are abundantly expressed in the ovary. In situ hybridization histochemistry using ovaries from gonadotropin-treated immature rats and regularly cycling adult rats show that this transcript is predominantly localized to granulosa cells of all healthy follicles, including primary follicles, and to newly-formed and healthy corpora lutea. This cell-specific expression pattern of the IAP-LE gene is distinct from those of the several known retroviral elements, suggesting the potentially novel functional importance of the IAP-LE gene. Taken together, our results demonstrate abundant and cell-specific expression of a novel IAP-LE in rat granulosa cells. (C) 2000 Academic Press.
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U2 - 10.1006/bbrc.2000.3759
DO - 10.1006/bbrc.2000.3759
M3 - Article
C2 - 11071854
AN - SCOPUS:0034638636
SN - 0006-291X
VL - 278
SP - 48
EP - 57
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -