Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated

Waren N. Baticados, William H. Witola, Noboru Inoue, Jung Yeon Kim, Noritaka Kuboki, Xuenan Xuan, Naoaki Yokoyama, Chihiro Sugimoto

Research output: Research - peer-reviewArticle

Abstract

During the attempt to seek T. congolense species-specific diagnostic antigens, we discovered one cDNA clone (P74) encoding 74 kDa putative abc1 protein (p74) from T. congolense PCF cDNA library. It has been suggested that members of the abc1 family are novel chaperonins and essential for both the mitochondrial electron transfer in the bc 1 complex and the coenzyme Q biosynthesis. Although abc1 protein in yeast has a nuclear or mitochondrial subcellular location, neither nuclear localization signal nor mitochondrial targeting signal was found within p74. Northern blot analysis revealed that the transcription level of P74 mRNA in bloodstream form (BSF) cells were 4 times higher than that in procyclic form cells. Western blot analysis also indicated that p74 was only expressed in T. congolense BSF cells, and revealed that molecular mass of native p74 was not 74 kDa but 56 kDa. This indicates extensive post-translational modification in p74. Although further characterization of p74 will be required, our findings provide implications for CoQ biosynthesis pathway in T. congolense.

LanguageEnglish (US)
Pages157-164
Number of pages8
JournalJournal of Veterinary Medical Science
Volume67
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

Fingerprint

Trypanosoma congolense
gene expression
proteins
Gene Expression
Proteins
Protein
Gene
cells
cDNA
bloodstream forms
biosynthesis
Diagnostics
Pathway
Targeting
Localization
procyclic forms
chaperonins
nuclear localization signals
ubiquinones
post-translational modification

Keywords

  • Abc1 family protein
  • Coenzyme Q
  • Trypanosoma congolense

ASJC Scopus subject areas

  • Marketing
  • veterinary(all)

Cite this

Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated. / Baticados, Waren N.; Witola, William H.; Inoue, Noboru; Kim, Jung Yeon; Kuboki, Noritaka; Xuan, Xuenan; Yokoyama, Naoaki; Sugimoto, Chihiro.

In: Journal of Veterinary Medical Science, Vol. 67, No. 2, 02.2005, p. 157-164.

Research output: Research - peer-reviewArticle

Baticados, WN, Witola, WH, Inoue, N, Kim, JY, Kuboki, N, Xuan, X, Yokoyama, N & Sugimoto, C 2005, 'Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated' Journal of Veterinary Medical Science, vol 67, no. 2, pp. 157-164. DOI: 10.1292/jvms.67.157
Baticados, Waren N. ; Witola, William H. ; Inoue, Noboru ; Kim, Jung Yeon ; Kuboki, Noritaka ; Xuan, Xuenan ; Yokoyama, Naoaki ; Sugimoto, Chihiro. / Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated. In: Journal of Veterinary Medical Science. 2005 ; Vol. 67, No. 2. pp. 157-164
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