Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated

Waren N. Baticados, William H. Witola, Noboru Inoue, Jung Yeon Kim, Noritaka Kuboki, Xuenan Xuan, Naoaki Yokoyama, Chihiro Sugimoto

Research output: Contribution to journalArticle

Abstract

During the attempt to seek T. congolense species-specific diagnostic antigens, we discovered one cDNA clone (P74) encoding 74 kDa putative abc1 protein (p74) from T. congolense PCF cDNA library. It has been suggested that members of the abc1 family are novel chaperonins and essential for both the mitochondrial electron transfer in the bc 1 complex and the coenzyme Q biosynthesis. Although abc1 protein in yeast has a nuclear or mitochondrial subcellular location, neither nuclear localization signal nor mitochondrial targeting signal was found within p74. Northern blot analysis revealed that the transcription level of P74 mRNA in bloodstream form (BSF) cells were 4 times higher than that in procyclic form cells. Western blot analysis also indicated that p74 was only expressed in T. congolense BSF cells, and revealed that molecular mass of native p74 was not 74 kDa but 56 kDa. This indicates extensive post-translational modification in p74. Although further characterization of p74 will be required, our findings provide implications for CoQ biosynthesis pathway in T. congolense.

Original languageEnglish (US)
Pages (from-to)157-164
Number of pages8
JournalJournal of Veterinary Medical Science
Volume67
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

Fingerprint

Trypanosoma congolense
Gene Expression
Proteins
Protein
proteins
cells
bloodstream forms
biosynthesis
Pathway
Gene
Diagnostics
Localization
Chaperonins
Nuclear Localization Signals
Ubiquinone
Fungal Proteins
Post Translational Protein Processing
Gene Library
Northern Blotting
Complementary DNA

Keywords

  • Abc1 family protein
  • Coenzyme Q
  • Trypanosoma congolense

ASJC Scopus subject areas

  • Marketing
  • veterinary(all)

Cite this

Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated. / Baticados, Waren N.; Witola, William H.; Inoue, Noboru; Kim, Jung Yeon; Kuboki, Noritaka; Xuan, Xuenan; Yokoyama, Naoaki; Sugimoto, Chihiro.

In: Journal of Veterinary Medical Science, Vol. 67, No. 2, 02.2005, p. 157-164.

Research output: Contribution to journalArticle

Baticados, WN, Witola, WH, Inoue, N, Kim, JY, Kuboki, N, Xuan, X, Yokoyama, N & Sugimoto, C 2005, 'Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated' Journal of Veterinary Medical Science, vol 67, no. 2, pp. 157-164. DOI: 10.1292/jvms.67.157

Baticados, Waren N.; Witola, William H.; Inoue, Noboru; Kim, Jung Yeon; Kuboki, Noritaka; Xuan, Xuenan; Yokoyama, Naoaki; Sugimoto, Chihiro / Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated.

In: Journal of Veterinary Medical Science, Vol. 67, No. 2, 02.2005, p. 157-164.

Research output: Contribution to journalArticle

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