Expression in transgenic plants of a viral gene product that mediates insect transmission of potyviruses.

P. H. Berger, A. G. Hunt, L. L. Domier, G. M. Hellmann, Y. Stram, D. W. Thornbury, T. P. Pirone

Research output: Contribution to journalArticle

Abstract

Helper component (HC) is a virus-encoded nonstructural protein that is required for transmission of potyviruses by their aphid vectors. As a prelude to studies on the molecular basis of HC activity, a cDNA clone (pPB-3) was constructed that contained the first three cistrons (34 kDa-HC-42 kDa) of the RNA genome of the potyvirus tobacco vein mottling virus, the first six nucleotides of the adjacent cylindrical inclusion body protein cistron, and a synthetic translation termination codon. This construction was introduced into tobacco cells via a Ti plasmid-based vector. Northern blot analysis of transgenic plants demonstrated the presence of an RNA of the size expected from the construction of pPB-3, and Western blot analysis revealed the presence of a protein that comigrated with authentic HC, indicating that the proteolytic activity necessary to produce mature-sized HC was encoded by pPB-3. The HC produced in the transgenic plants was demonstrated to be active in a virus transmission bioassay with aphids.

Original languageEnglish (US)
Pages (from-to)8402-8406
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number21
DOIs
StatePublished - Nov 1989
Externally publishedYes

ASJC Scopus subject areas

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