Expression and immunolocalization of a Boophilus microplus cathepsin L-like enzyme

Gaby Renard, Flávio Alves Lara, Felipe Cardoso De Cardoso, Flávio Costa Miguens, Marílvia Dansa-Petretski, Carlos Termignoni, Aoi Masuda

Research output: Contribution to journalArticle

Abstract

Efforts are being undertaken to control tick infestations that cause important economic losses. A cathepsin L-like endopeptidase of Boophilus microplus was expressed in Escherichia coli; the recombinant enzyme was capable of hydrolysing gelatin, tick vitellin and bovine haemoglobin. In this paper we focus on the expression and local of synthesis of this enzyme in the tick. RT-PCR experiments showed that this endopeptidase is transcribed in the gut of partially engorged tick females. In immunoblotting, polyclonal antibodies against the recombinant enzyme reacted with proteins of larvae older than 5 days, of fully and partially engorged female gut. In immunolocalization experiments the enzyme was localized in probable secretory cells of the gut. Based on our findings we postulate that BmCL1 may be involved in haemoglobin degradation in the B. microplus gut. This enzyme may be used as target for the control of this parasite.

Original languageEnglish (US)
Pages (from-to)325-328
Number of pages4
JournalInsect Molecular Biology
Volume11
Issue number4
DOIs
StatePublished - Aug 24 2002
Externally publishedYes

Keywords

  • Boophilus microplus
  • Cathepsin L
  • Cysteine protease
  • Gut
  • Tick

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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  • Cite this

    Renard, G., Lara, F. A., De Cardoso, F. C., Miguens, F. C., Dansa-Petretski, M., Termignoni, C., & Masuda, A. (2002). Expression and immunolocalization of a Boophilus microplus cathepsin L-like enzyme. Insect Molecular Biology, 11(4), 325-328. https://doi.org/10.1046/j.1365-2583.2002.00342.x