TY - JOUR
T1 - Expression and comparison of sweet corn CYP81A9s in relation to nicosulfuron sensitivity
AU - Choe, Eunsoo
AU - Williams, Martin M.
N1 - Funding Information:
The authors thank Jim Moody, Nick Hausman, Laura Crawford, Joseph Kibiwott, and several undergraduate students for proving technical support and assistance on the greenhouse experiment. We also thank Dr. Steven Huber, plant physiologist (retired) at Global Change and Photosynthesis Research unit, USDA-ARS, for providing support and reviewing the manuscript, and Dr. Mary A. Schuler, professor at the department of cell and developmental biology, University of Illinois at Urbana-Champaign for providing guidance for expressing, characterizing and interpreting corn P450s. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable.
Publisher Copyright:
© 2020 Society of Chemical Industry
PY - 2020/9/1
Y1 - 2020/9/1
N2 - BACKGROUND: Nicosulfuron, a sulfonylurea herbicide widely used for grass weed control in corn production, injures some sweet corn hybrids and inbreds. A specific cytochrome P450 (P450), CYP81A9, is suggested to be responsible for sensitivity to nicosulfuron and other P450-metabolized herbicides. Corn CYP81A9 enzymes were expressed in E. coli and investigated to find the factor(s) associated with their function and variation in metabolizing nicosulfuron. RESULT: Recombinant expressed CYP81A9s from tolerant sweet corn inbreds produced an active form of P450, while CYP81A9 from a sensitive inbred produced an inactive form. Nicosulfuron bound to tolerant CYP81A9s, and produced reverse-type I ligand, while sensitive CYP81A9 showed no interaction with nicosulfuron. Investigation of 106 sweet corn inbreds showed variation in nicosulfuron injury. A survey of sweet corn CYP81A9 sequences showed mutations in codons for amino acids at 269, 284, 375, and 477 occurred in sweet corn inbreds with complete loss of P450 function (with mean injury >91%) and amino acid changes at 208 and 472 occurred in inbreds with moderate and complete loss of P450 function (with mean injury >14%). CONCLUSION: Our results support that CYP81A9 enzyme is responsible for metabolizing nicosulfuron in sweet corn, and different types of amino acid changes in CYP81A9 sequence are associated with variation in nicosulfuron injury. Therefore, a careful selection of the tolerant allele will be critical for improving tolerance to nicosulfuron and several other P450-metabolized herbicides.
AB - BACKGROUND: Nicosulfuron, a sulfonylurea herbicide widely used for grass weed control in corn production, injures some sweet corn hybrids and inbreds. A specific cytochrome P450 (P450), CYP81A9, is suggested to be responsible for sensitivity to nicosulfuron and other P450-metabolized herbicides. Corn CYP81A9 enzymes were expressed in E. coli and investigated to find the factor(s) associated with their function and variation in metabolizing nicosulfuron. RESULT: Recombinant expressed CYP81A9s from tolerant sweet corn inbreds produced an active form of P450, while CYP81A9 from a sensitive inbred produced an inactive form. Nicosulfuron bound to tolerant CYP81A9s, and produced reverse-type I ligand, while sensitive CYP81A9 showed no interaction with nicosulfuron. Investigation of 106 sweet corn inbreds showed variation in nicosulfuron injury. A survey of sweet corn CYP81A9 sequences showed mutations in codons for amino acids at 269, 284, 375, and 477 occurred in sweet corn inbreds with complete loss of P450 function (with mean injury >91%) and amino acid changes at 208 and 472 occurred in inbreds with moderate and complete loss of P450 function (with mean injury >14%). CONCLUSION: Our results support that CYP81A9 enzyme is responsible for metabolizing nicosulfuron in sweet corn, and different types of amino acid changes in CYP81A9 sequence are associated with variation in nicosulfuron injury. Therefore, a careful selection of the tolerant allele will be critical for improving tolerance to nicosulfuron and several other P450-metabolized herbicides.
KW - CYP81A9
KW - P450-metabolized herbicide
KW - Zea mays
KW - cytochrome P450
KW - nicosulfuron herbicide
KW - nsf1
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U2 - 10.1002/ps.5848
DO - 10.1002/ps.5848
M3 - Article
C2 - 32248609
AN - SCOPUS:85084213371
SN - 1526-498X
VL - 76
SP - 3012
EP - 3019
JO - Pest Management Science
JF - Pest Management Science
IS - 9
ER -