Expression and activity of glycogen synthase kinase during vitellogenesis and embryogenesis of Rhipicephalus (Boophilus) microplus

Carlos Logullo, William H. Witola, Caroline Andrade, Leonardo Abreu, Josiana Gomes, Itabajara da Silva Vaz, Saiki Imamura, Satoru Konnai, Kazuhiko Ohashi, Misao Onuma

Research output: Research - peer-reviewArticle

Abstract

Glycogen synthase kinase 3 (GSK-3) is classically described as a key enzyme involved in glycogen metabolism in mammals. GSK-3 belongs to a highly conserved family of serine/threonine protein kinases, whose members are involved in hormonal regulation, nuclear signaling, and cell fate determination in higher eukaryotes. We have cloned and characterized the RmGSK-3 gene from Rhipicephalus (Boophilus) microplus tick embryos. DNA and protein sequence analysis depicted high similarity to the corresponding enzyme, from both vertebrate and invertebrate animals. In addition, the mRNA transcription profile identified during embryogenesis was analyzed. We observed that the RmGSK-3 mRNA rapidly decreases from the 1st to 3rd day of development, and increases from the 3rd to 15th day. After the 15th day of development, we observed a near 50% reduction in RmGSK-3 mRNA transcription in comparison to the 1st day. We detected the GSK-3β isoform in egg homogenates throughout embryogenesis using Western blot analysis. RmGSK-3 mRNA was present in fat body, midgut and ovary from partially and fully engorged adult female ticks. The highest mRNA level was observed in ovaries from both developmental stages and in first-day eggs. Furthermore, RmGSK-3 activity correlated with glycogen content variation. Finally, kinase activity in egg homogenates was inhibited by the specific inhibitor, SB-216763. These data suggest that RmGSK-3β may be involved in glycogen metabolism regulation during R. microplus embryogenesis.

LanguageEnglish (US)
Pages261-269
Number of pages9
JournalVeterinary Parasitology
Volume161
Issue number3-4
DOIs
StatePublished - May 12 2009
Externally publishedYes

Fingerprint

Rhipicephalus microplus
vitellogenesis
glycogen
embryogenesis
glycogen synthase kinases
Rhipicephalus
Vitellogenesis
Glycogen Synthase Kinases
Embryonic Development
Messenger RNA
ticks
transcription (genetics)
metabolism
enzymes
tau-protein kinase
Glycogen
Glycogen Synthase Kinase 3
Ticks
Ovum
Ovary

Keywords

  • Embryogenesis
  • Glycogen synthase kinase
  • Metabolism
  • Rhipicephalus (Boophilus) microplus
  • Tick egg

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Cite this

Expression and activity of glycogen synthase kinase during vitellogenesis and embryogenesis of Rhipicephalus (Boophilus) microplus. / Logullo, Carlos; Witola, William H.; Andrade, Caroline; Abreu, Leonardo; Gomes, Josiana; da Silva Vaz, Itabajara; Imamura, Saiki; Konnai, Satoru; Ohashi, Kazuhiko; Onuma, Misao.

In: Veterinary Parasitology, Vol. 161, No. 3-4, 12.05.2009, p. 261-269.

Research output: Research - peer-reviewArticle

Logullo, C, Witola, WH, Andrade, C, Abreu, L, Gomes, J, da Silva Vaz, I, Imamura, S, Konnai, S, Ohashi, K & Onuma, M 2009, 'Expression and activity of glycogen synthase kinase during vitellogenesis and embryogenesis of Rhipicephalus (Boophilus) microplus' Veterinary Parasitology, vol 161, no. 3-4, pp. 261-269. DOI: 10.1016/j.vetpar.2009.01.029
Logullo, Carlos ; Witola, William H. ; Andrade, Caroline ; Abreu, Leonardo ; Gomes, Josiana ; da Silva Vaz, Itabajara ; Imamura, Saiki ; Konnai, Satoru ; Ohashi, Kazuhiko ; Onuma, Misao. / Expression and activity of glycogen synthase kinase during vitellogenesis and embryogenesis of Rhipicephalus (Boophilus) microplus. In: Veterinary Parasitology. 2009 ; Vol. 161, No. 3-4. pp. 261-269
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