TY - JOUR
T1 - Exploring Biliverdin’s Molecular Interactions with Cu- and Fe-Based MOFs
T2 - A Unified In Vitro Study with Photoacoustic Analysis
AU - R. Alanagh, Hamideh
AU - Fathi, Parinaz
AU - Knox, Hailey J.
AU - Moitra, Parikshit
AU - Chan, Jefferson
AU - Pan, Dipanjan
N1 - This work was carried out in part in the Frederick Seitz Materials Research Laboratory Central Research Facilities, University of Illinois. We gratefully acknowledge the Chemical and Biomolecular Engineering department shared facilities for access to their fluorescence spectrometer. This project was funded through grants from National Institute of Health, Department of Defense, and University of Illinois. P.F. was supported by the National Physical Science Consortium and the National Institute of Standards & Technology through an NPSC graduate fellowship and by the Nadine Barrie Smith Memorial Fellowship from the Beckman Institute. Research reported in this publication was supported by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under award no. T32EB019944. We appreciate the support of Prof. Matthew J. Harrington from McGill University in completing BET and XRD characterizations.
PY - 2024/9/3
Y1 - 2024/9/3
N2 - Metal-organic frameworks (MOFs) have shown promise in enhancing the stability of biomolecules. Herein, biliverdin (BVD), a photoacoustic (PA) and fluorescent agent, was immobilized within the pores of NH2-MIL-101 (Fe) (FeMOFs) and on the surface of CuBTC crystallites (CuMOFs). MOFs were found to enhance the fluorescence emission and quench the PA intensity of biliverdin. Fluorescence and PA studies, in tandem with DFT simulations, demonstrated that the spectral interactions between MOFs and BVD resulted from interactions between biliverdin and the MOF pores and surfaces in addition to alterations in the HOMO-LUMO energy gap. The MOF internal structure of the MOF played a role in BVD loading, with the FeMOFs enabling greater BVD encapsulation, while CuMOF interactions with BVD primarily took place on the MOF surface. The role of these surface vs pore interactions in the release of biliverdin was explored. This study demonstrates that the effects of the MOF internal structure, surface interactions, and energy interactions should be taken into consideration for biomolecule loading in MOFs.
AB - Metal-organic frameworks (MOFs) have shown promise in enhancing the stability of biomolecules. Herein, biliverdin (BVD), a photoacoustic (PA) and fluorescent agent, was immobilized within the pores of NH2-MIL-101 (Fe) (FeMOFs) and on the surface of CuBTC crystallites (CuMOFs). MOFs were found to enhance the fluorescence emission and quench the PA intensity of biliverdin. Fluorescence and PA studies, in tandem with DFT simulations, demonstrated that the spectral interactions between MOFs and BVD resulted from interactions between biliverdin and the MOF pores and surfaces in addition to alterations in the HOMO-LUMO energy gap. The MOF internal structure of the MOF played a role in BVD loading, with the FeMOFs enabling greater BVD encapsulation, while CuMOF interactions with BVD primarily took place on the MOF surface. The role of these surface vs pore interactions in the release of biliverdin was explored. This study demonstrates that the effects of the MOF internal structure, surface interactions, and energy interactions should be taken into consideration for biomolecule loading in MOFs.
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U2 - 10.1021/acs.langmuir.3c03584
DO - 10.1021/acs.langmuir.3c03584
M3 - Article
C2 - 39167692
AN - SCOPUS:85201769027
SN - 0743-7463
VL - 40
SP - 18407
EP - 18417
JO - Langmuir
JF - Langmuir
IS - 35
ER -