Abstract
We present the first experimental demonstration of two-photon Mueller matrix second-harmonic generation (SHG) microscopy for the extraction of representative Mueller matrices. Individual SHG images of porcine sample sets are divided into subimages, and the local nonlinear two-photon Mueller matrices (M(2)) and degree of polarization (p) are determined. Results of the two-photon Mueller matrices from different sample types and the effects of thickness on the mean p distribution per polarization basis are investigated. We find that the p distribution shape changes and an associated bimodal mean difference (μd) increases with thickness. Our approach has potential as a quantitative imaging technique and can be applied to other multiphoton coherent imaging modalities.
Original language | English (US) |
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Article number | 016011 |
Journal | Journal of biomedical optics |
Volume | 21 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 2016 |
Keywords
- microscopy
- nonlinear optics
- polarimetry
- second-harmonic generation
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering