Experimental demonstration of two-photon Mueller matrix second-harmonic generation microscopy

Chukwuemeka Okoro, Kimani C. Toussaint

Research output: Contribution to journalArticle

Abstract

We present the first experimental demonstration of two-photon Mueller matrix second-harmonic generation (SHG) microscopy for the extraction of representative Mueller matrices. Individual SHG images of porcine sample sets are divided into subimages, and the local nonlinear two-photon Mueller matrices (M(2)) and degree of polarization (p) are determined. Results of the two-photon Mueller matrices from different sample types and the effects of thickness on the mean p distribution per polarization basis are investigated. We find that the p distribution shape changes and an associated bimodal mean difference (μd) increases with thickness. Our approach has potential as a quantitative imaging technique and can be applied to other multiphoton coherent imaging modalities.

Original languageEnglish (US)
Article number016011
JournalJournal of biomedical optics
Volume21
Issue number1
DOIs
StatePublished - Jan 1 2016

Keywords

  • microscopy
  • nonlinear optics
  • polarimetry
  • second-harmonic generation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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