Experimental and computational determination of tRNA dynamics

Rebecca W. Alexander; John Eargle; Zaida Luthey-Schulten

doi:10.1016/j.febslet.2009.11.061

Experimental and computational determination of tRNA dynamics

Rebecca W. Alexander, John Eargle, Zaida Luthey-Schulten

Research output: Contribution to journal › Review article › peer-review

Abstract

As the molecular representation of the genetic code, tRNA plays a central role in the translational machinery where it interacts with several proteins and other RNAs during the course of protein synthesis. These interactions exploit the dynamic flexibility of tRNA. In this minireview, we discuss the effects of modified bases, ions, and proteins on tRNA structure and dynamics and the challenges of observing its motions over the cycle of translation.

Original language	English (US)
Pages (from-to)	376-386
Number of pages	11
Journal	FEBS Letters
Volume	584
Issue number	2
DOIs	https://doi.org/10.1016/j.febslet.2009.11.061
State	Published - Jan 21 2010

Keywords

Conformational change
Flexibility
Induced fit
Molecular dynamics
Translation
tRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Online availability

10.1016/j.febslet.2009.11.061

Library availability

Discover UIUC Full Text

Cite this

@article{c74943d9fdfa49e59bc46a710e37f889,

title = "Experimental and computational determination of tRNA dynamics",

abstract = "As the molecular representation of the genetic code, tRNA plays a central role in the translational machinery where it interacts with several proteins and other RNAs during the course of protein synthesis. These interactions exploit the dynamic flexibility of tRNA. In this minireview, we discuss the effects of modified bases, ions, and proteins on tRNA structure and dynamics and the challenges of observing its motions over the cycle of translation.",

keywords = "Conformational change, Flexibility, Induced fit, Molecular dynamics, Translation, tRNA",

author = "Alexander, {Rebecca W.} and John Eargle and Zaida Luthey-Schulten",

note = "Funding Information: The authors are supported by NSF Grants MCB-0448243 (R.W.A.), MCB-0844670 (Z.L.S.) and PHY-0822613 (J.E.) and by a fellowship from the National Foundation for Cancer Research (R.W.A.). We thank Alexis Black Pyrkosz for creating the sequence entropy and tRNA cloverleaf diagrams.",

year = "2010",

month = jan,

day = "21",

doi = "10.1016/j.febslet.2009.11.061",

language = "English (US)",

volume = "584",

pages = "376--386",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley & Sons, Ltd.",

number = "2",

}

TY - JOUR

T1 - Experimental and computational determination of tRNA dynamics

AU - Alexander, Rebecca W.

AU - Eargle, John

AU - Luthey-Schulten, Zaida

N1 - Funding Information: The authors are supported by NSF Grants MCB-0448243 (R.W.A.), MCB-0844670 (Z.L.S.) and PHY-0822613 (J.E.) and by a fellowship from the National Foundation for Cancer Research (R.W.A.). We thank Alexis Black Pyrkosz for creating the sequence entropy and tRNA cloverleaf diagrams.

PY - 2010/1/21

Y1 - 2010/1/21

N2 - As the molecular representation of the genetic code, tRNA plays a central role in the translational machinery where it interacts with several proteins and other RNAs during the course of protein synthesis. These interactions exploit the dynamic flexibility of tRNA. In this minireview, we discuss the effects of modified bases, ions, and proteins on tRNA structure and dynamics and the challenges of observing its motions over the cycle of translation.

AB - As the molecular representation of the genetic code, tRNA plays a central role in the translational machinery where it interacts with several proteins and other RNAs during the course of protein synthesis. These interactions exploit the dynamic flexibility of tRNA. In this minireview, we discuss the effects of modified bases, ions, and proteins on tRNA structure and dynamics and the challenges of observing its motions over the cycle of translation.

KW - Conformational change

KW - Flexibility

KW - Induced fit

KW - Molecular dynamics

KW - Translation

KW - tRNA

UR - http://www.scopus.com/inward/record.url?scp=71549156528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71549156528&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2009.11.061

DO - 10.1016/j.febslet.2009.11.061

M3 - Review article

C2 - 19932098

AN - SCOPUS:71549156528

SN - 0014-5793

VL - 584

SP - 376

EP - 386

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Experimental and computational determination of tRNA dynamics

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this