Expanding the boundary of biocatalysis: design and optimization of in vitro tandem catalytic reactions for biochemical production

Yajie Wang, Hengqian Ren, Huimin Zhao

Research output: Contribution to journalReview article


Biocatalysts have been increasingly used in the synthesis of fine chemicals and medicinal compounds due to significant advances in enzyme discovery and engineering. To mimic the synergistic effects of cascade reactions catalyzed by multiple enzymes in nature, researchers have been developing artificial tandem enzymatic reactions in vivo by harnessing synthetic biology and metabolic engineering tools. There is also growing interest in the development of one-pot tandem enzymatic or chemo-enzymatic processes in vitro due to their neat and concise catalytic systems and product purification procedures. In this review, we will briefly summarize the strategies of designing and optimizing in vitro tandem catalytic reactions, highlight a few representative examples, and discuss the future trend in this field.

Original languageEnglish (US)
Pages (from-to)115-129
Number of pages15
JournalCritical Reviews in Biochemistry and Molecular Biology
Issue number2
StatePublished - Mar 4 2018



  • Enzymatic
  • chemoenzymatic
  • in vitro
  • multistep
  • tandem

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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