Exercise training-induced modification of the gut microbiota persists after microbiota colonization and attenuates the response to chemically-induced colitis in gnotobiotic mice

J. M. Allen, L. J. Mailing, J. Cohrs, C. Salmonson, J. D. Fryer, V. Nehra, V. L. Hale, P. Kashyap, B. A. White, Jeffrey A Woods

Research output: Contribution to journalArticle

Abstract

Exercise reduces the risk of inflammatory disease by modulating a variety of tissue and cell types, including those within the gastrointestinal tract. Recent data indicates that exercise can also alter the gut microbiota, but little is known as to whether these changes affect host function. Here, we use a germ-free (GF) animal model to test whether exercise-induced modifications in the gut microbiota can directly affect host responses to microbiota colonization and chemically-induced colitis. Donor mice (n = 19) received access to a running wheel (n = 10) or remained without access (n = 9) for a period of six weeks. After euthanasia, cecal contents were pooled by activity treatment and transplanted into two separate cohorts of GF mice. Two experiments were then conducted. First, mice were euthanized five weeks after the microbiota transplant and tissues were collected for analysis. A second cohort of GF mice were colonized by donor microbiotas for four weeks before dextran-sodium-sulfate was administered to induce acute colitis, after which mice were euthanized for tissue analysis. We observed that microbial transplants from donor (exercised or control) mice led to differences in microbiota β-diversity, metabolite profiles, colon inflammation, and body mass in recipient mice five weeks after colonization. We also demonstrate that colonization of mice with a gut microbiota from exercise-trained mice led to an attenuated response to chemical colitis, evidenced by reduced colon shortening, attenuated mucus depletion and augmented expression of cytokines involved in tissue regeneration. Exercise-induced modifications in the gut microbiota can mediate host-microbial interactions with potentially beneficial outcomes for the host.

Original languageEnglish (US)
Pages (from-to)115-130
Number of pages16
JournalGut Microbes
Volume9
Issue number2
DOIs
StatePublished - Mar 4 2018

Fingerprint

Germ-Free Life
Microbiota
Colitis
Exercise
Tissue Donors
Colon
Microbial Interactions
Gastrointestinal Microbiome
Dextran Sulfate
Euthanasia
Mucus
Exercise Test
Running
Gastrointestinal Tract
Regeneration
Animal Models
Cytokines
Inflammation
Transplants

Keywords

  • colitis
  • colonization inflammation
  • exercise
  • germ-free
  • gut
  • microbiome
  • microbiota
  • transplant
  • voluntary wheel running

ASJC Scopus subject areas

  • Microbiology
  • Gastroenterology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Exercise training-induced modification of the gut microbiota persists after microbiota colonization and attenuates the response to chemically-induced colitis in gnotobiotic mice. / Allen, J. M.; Mailing, L. J.; Cohrs, J.; Salmonson, C.; Fryer, J. D.; Nehra, V.; Hale, V. L.; Kashyap, P.; White, B. A.; Woods, Jeffrey A.

In: Gut Microbes, Vol. 9, No. 2, 04.03.2018, p. 115-130.

Research output: Contribution to journalArticle

Allen, J. M. ; Mailing, L. J. ; Cohrs, J. ; Salmonson, C. ; Fryer, J. D. ; Nehra, V. ; Hale, V. L. ; Kashyap, P. ; White, B. A. ; Woods, Jeffrey A. / Exercise training-induced modification of the gut microbiota persists after microbiota colonization and attenuates the response to chemically-induced colitis in gnotobiotic mice. In: Gut Microbes. 2018 ; Vol. 9, No. 2. pp. 115-130.
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