Exercise speeds cutaneous wound healing in high-fat diet-induced obese mice

Brandt D. Pence, Luisa A. Dipietro, Jeffrey A. Woods

Research output: Contribution to journalArticle

Abstract

PURPOSE: Obesity has been shown to impair cutaneous wound healing, which is associated with increased wound inflammation. Exercise is known to decrease obesity-associated inflammation and has been shown to speed cutaneous wound healing in aged mice. Therefore, we investigated whether treadmill exercise could speed cutaneous wound healing in obese, high-fat diet-fed mice. METHODS: We fed female C57Bl/6J mice a high-fat diet (45% calories from fat) for 16 wk to induce a state of obesity and insulin resistance. Mice then ran on a treadmill for 3 d before excisional wounding. On day 4, mice were wounded 1 h after exercise. Mice then exercised for 5 d after wounding, and healing was assessed by photoplanimetry for 10 d. RESULTS: As described previously, obesity impaired wound healing, with significantly larger wound sizes measured from days 3 to day 10 after wounding (P < 0.05). Exercise did not improve healing in lean mice fed a normal chow diet. However, wound size was significantly smaller in exercised obese mice compared with their lean counterparts (P < 0.05 at day 1, day 4, and day 5 after wound). Surprisingly, we were unable to detect any differences in gene or protein expression of proinflammatory cytokines interleukin-1β and tumor necrosis factor-α or the anti-inflammatory cytokine interleukin-10 in the wounds. Likewise, there were no differences in gene expression of chemokines monocyte chemoattractant protein-1 and keratinocyte chemoattractant or of growth factor platelet-derived growth factor in wounds of exercise and sedentary mice. CONCLUSION: This suggests an effect of exercise independent of alterations in inflammation. Future work should focus on early events after wounding, including exercise effects on hemostasis and myofibroblast function.

Original languageEnglish (US)
Pages (from-to)1846-1854
Number of pages9
JournalMedicine and Science in Sports and Exercise
Volume44
Issue number10
DOIs
StatePublished - Oct 1 2012

Fingerprint

Obese Mice
High Fat Diet
Wound Healing
Skin
Wounds and Injuries
Obesity
Inflammation
Cytokines
Myofibroblasts
Chemokine CCL2
Platelet-Derived Growth Factor
Chemotactic Factors
Hemostasis
Interleukin-1
Keratinocytes
Chemokines
Interleukin-10
Insulin Resistance
Intercellular Signaling Peptides and Proteins
Anti-Inflammatory Agents

Keywords

  • Obesity
  • inflammation
  • mice
  • training

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Exercise speeds cutaneous wound healing in high-fat diet-induced obese mice. / Pence, Brandt D.; Dipietro, Luisa A.; Woods, Jeffrey A.

In: Medicine and Science in Sports and Exercise, Vol. 44, No. 10, 01.10.2012, p. 1846-1854.

Research output: Contribution to journalArticle

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