Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer

Lee W. Jones, Jodi Antonelli, Elizabeth M. Masko, Gloria Broadwater, Christopher D. Lascola, Diane Fels, Mark W. Dewhirst, Jason R.B. Dyck, Jeevan Nagendran, Catherine T. Flores, Allison S. Betof, Erik R. Nelson, Michael Pollak, Rajesh C. Dash, Martin E. Young, Stephen J. Freedland

Research output: Contribution to journalArticle

Abstract

The purpose of this study is to investigate the effects of exercise on cancer progression, metastasis, and underlying mechanisms in an orthotopic model of murine prostate cancer. C57BL/6 male mice (6-8 wk of age) were orthotopically injected with transgenic adenocarcinoma of mouse prostate C-1 cells (5 × 105) and randomly assigned to exercise (n = 28) or a non-intervention control (n = 31) groups. The exercise group was given voluntary access to a wheel 24 h/day for the duration of the study. Four mice per group were serially killed on days 14, 31, and 36; the remaining 38 mice (exercise, n = 18; control, n = 20) were killed on day 53. Before death, MRI was performed to assess tumor blood perfusion. Primary tumor growth rate was comparable between groups, but expression of prometastatic genes was significantly modulated in exercising animals with a shift toward reduced metastasis. Exercise was associated with increased activity of protein kinases within the MEK/MAPK and PI3K/mTOR signaling cascades with subsequent increased intratumoral protein levels of HIF-1α and VEGF. This was associated with improved tumor vascularization. Multiplex ELISAs revealed distinct reductions in plasma concentrations of several angiogenic cytokines in the exercise group, which was associated with increased expression of angiogenic and metabolic genes in the skeletal muscle. Exercise-induced stabilization of HIF-1α and subsequent upregulation of VEGF was associated with "productive" tumor vascularization with a shift toward suppressed metastasis in an orthotopic model of prostate cancer.

Original languageEnglish (US)
Pages (from-to)263-272
Number of pages10
JournalJournal of Applied Physiology
Volume113
Issue number2
DOIs
StatePublished - Jul 15 2012
Externally publishedYes

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Prostatic Neoplasms
Neoplasms
Neoplasm Metastasis
Vascular Endothelial Growth Factor A
Mitogen-Activated Protein Kinase Kinases
Phosphatidylinositol 3-Kinases
Protein Kinases
Transgenic Mice
Prostate
Skeletal Muscle
Adenocarcinoma
Up-Regulation
Perfusion
Enzyme-Linked Immunosorbent Assay
Cytokines
Gene Expression
Growth
Genes
Proteins

Keywords

  • Exercise
  • Prostrate cancer

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Jones, L. W., Antonelli, J., Masko, E. M., Broadwater, G., Lascola, C. D., Fels, D., ... Freedland, S. J. (2012). Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer. Journal of Applied Physiology, 113(2), 263-272. https://doi.org/10.1152/japplphysiol.01575.2011

Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer. / Jones, Lee W.; Antonelli, Jodi; Masko, Elizabeth M.; Broadwater, Gloria; Lascola, Christopher D.; Fels, Diane; Dewhirst, Mark W.; Dyck, Jason R.B.; Nagendran, Jeevan; Flores, Catherine T.; Betof, Allison S.; Nelson, Erik R.; Pollak, Michael; Dash, Rajesh C.; Young, Martin E.; Freedland, Stephen J.

In: Journal of Applied Physiology, Vol. 113, No. 2, 15.07.2012, p. 263-272.

Research output: Contribution to journalArticle

Jones, LW, Antonelli, J, Masko, EM, Broadwater, G, Lascola, CD, Fels, D, Dewhirst, MW, Dyck, JRB, Nagendran, J, Flores, CT, Betof, AS, Nelson, ER, Pollak, M, Dash, RC, Young, ME & Freedland, SJ 2012, 'Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer', Journal of Applied Physiology, vol. 113, no. 2, pp. 263-272. https://doi.org/10.1152/japplphysiol.01575.2011
Jones, Lee W. ; Antonelli, Jodi ; Masko, Elizabeth M. ; Broadwater, Gloria ; Lascola, Christopher D. ; Fels, Diane ; Dewhirst, Mark W. ; Dyck, Jason R.B. ; Nagendran, Jeevan ; Flores, Catherine T. ; Betof, Allison S. ; Nelson, Erik R. ; Pollak, Michael ; Dash, Rajesh C. ; Young, Martin E. ; Freedland, Stephen J. / Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer. In: Journal of Applied Physiology. 2012 ; Vol. 113, No. 2. pp. 263-272.
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AU - Nelson, Erik R.

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