TY - JOUR
T1 - Examining Genetic Variation in Maize Inbreds and Mapping Oxidative Stress Response QTL in B73-Mo17 Nearly Isogenic Lines
AU - Sorgini, Crystal A.
AU - Barrios-Perez, Ilse
AU - Brown, Patrick J.
AU - Ainsworth, Elizabeth A.
N1 - Publisher Copyright:
© Copyright © 2019 Sorgini, Barrios-Perez, Brown and Ainsworth.
PY - 2019/7/4
Y1 - 2019/7/4
N2 - Screening crop plants under elevated ozone concentrations ([O3]) is a pre-requisite for identification of tolerant lines, but few studies have mapped maize responses to elevated [O3]. B73-Mo17 nearly isogenic lines (NILs) were screened in the field under ambient (~40 ppb) and elevated (~100 ppb) [O3] at the Free Air gas Concentration Enrichment (FACE) research facility in Champaign, IL to identify maize leaf damage QTL associated with variation in O3-induced oxidative stress response. In Mo17 NILs, a significant leaf damage QTL was identified at 161Mb on chromosome 2. To assess the feasibility of high-throughput phenotyping and fine mapping of early season O3 leaf damage QTL, a subset of the nested association mapping (NAM) founder lines were screened in a growth chamber experiment under ambient and elevated [O3]. Results showed that elevated [O3] decreased the number of green leaves while increasing the number of lesioned and dead leaves. Most lines showed the same general response to elevated [O3], but the degree of damage varied among lines. Next, tolerant and sensitive B73-Mo17 NILs identified from the FACE study, and hybrid crosses of the identified NILs with Mo17 (n = 20) were grown under elevated O3 (~150 ppb) in growth chambers (n = 7). In the chambers, O3-sensitive lines could be distinguished from tolerant lines based on leaf lesions, but there was not a continuous degree of damage like that seen in the field. This research identified a repeatable O3-induced leaf damage QTL and developed populations and markers that can be used in future growth chamber fine mapping experiments. These results demonstrate the feasibility of high-throughput phenotyping and fine mapping of O3 leaf damage QTL in a controlled environment.
AB - Screening crop plants under elevated ozone concentrations ([O3]) is a pre-requisite for identification of tolerant lines, but few studies have mapped maize responses to elevated [O3]. B73-Mo17 nearly isogenic lines (NILs) were screened in the field under ambient (~40 ppb) and elevated (~100 ppb) [O3] at the Free Air gas Concentration Enrichment (FACE) research facility in Champaign, IL to identify maize leaf damage QTL associated with variation in O3-induced oxidative stress response. In Mo17 NILs, a significant leaf damage QTL was identified at 161Mb on chromosome 2. To assess the feasibility of high-throughput phenotyping and fine mapping of early season O3 leaf damage QTL, a subset of the nested association mapping (NAM) founder lines were screened in a growth chamber experiment under ambient and elevated [O3]. Results showed that elevated [O3] decreased the number of green leaves while increasing the number of lesioned and dead leaves. Most lines showed the same general response to elevated [O3], but the degree of damage varied among lines. Next, tolerant and sensitive B73-Mo17 NILs identified from the FACE study, and hybrid crosses of the identified NILs with Mo17 (n = 20) were grown under elevated O3 (~150 ppb) in growth chambers (n = 7). In the chambers, O3-sensitive lines could be distinguished from tolerant lines based on leaf lesions, but there was not a continuous degree of damage like that seen in the field. This research identified a repeatable O3-induced leaf damage QTL and developed populations and markers that can be used in future growth chamber fine mapping experiments. These results demonstrate the feasibility of high-throughput phenotyping and fine mapping of O3 leaf damage QTL in a controlled environment.
KW - FACE technology
KW - QTL mapping
KW - global climate change
KW - maize
KW - tropospheric ozone (O)
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U2 - 10.3389/fsufs.2019.00051
DO - 10.3389/fsufs.2019.00051
M3 - Article
AN - SCOPUS:85077560988
SN - 2571-581X
VL - 3
JO - Frontiers in Sustainable Food Systems
JF - Frontiers in Sustainable Food Systems
M1 - 51
ER -